XB-CLONE-4352230
Clone Name: IMAGE:7027322
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Gene:
eef1a1o
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784384 [+]
taattaatccccccccccccctcgcagtctctgcctgctctgctgtatttgttcgtgggggttgactggtgttcgctgccaagatgggaaaggaaaagat
tcacattaacatcgttgtgatcgggcatgtggactctggcaagtcaaccacaaccgggcatctcatctacaaatgcgggggcattgacaaaaggaccatc
gaaaagtttgagaaggaagctgcagagatgggtaaaggctcctttaaatatgcttgggtcttggataagctgaaagccgagagagaacgtggcataacca
ttgatatttccttgtggaagttcgagactggaaaattctatatcacaattattgatgctcctgggcacagagacttcatcaaaaacatgatcactggcac
ttctcaggctgactgtgcagtgctcattgttgctggtggtgttggagaatttgaggctggtatctctaaaaatggacagactcgagagcatgccctctta
gctttcactctaggtgtcaagcagcttataattggagtaaacaagatggactccactgagccccctttcagccaaaaaagatttgaggaaattactaaag
aagtcagtgccctacattaagaagattggctacaacccagcgactgttccatttgtgccgatatctggatggcatggagacaacatgctggaggctagca
ccaatatgccctggttttaagggccggaacattgaaaaggaaaaaaggaaatgcccagtgggtgtcacacttgctagaaagcacttgaattgccattaat
tccttccctcagccgt
3' EST: CF784383 [+]tttttttttttttttctttttttttttttttttttttttttgcatcattaaacatttattcatttttacaggacaccaagattacactgtacagggttac
acagatcatttcttgccagcttttacagcagacttagtcacttttccagagcttgctgcctttttatccacacctttgattacgccaacagctaccgtct
gcctcatgtcacgcactgcaaatcggccaaggggtgggtagtctgaaaaggtttctacacacatgggcttcccaggaatcatttccactatagctgcatc
tccagatttcaagaattttgggtcatcctccagcttcttgccacttcttctgtctatcttttgcttcagctcagcaaacttacaggcaatatgagctgta
tggcagtccaggactggagcatagccagcactaatctgtcctgggtgattgagtataattacctgtgctgtaaaggttccagcttgcataggtggatcat
ttttgctatcaccagccacattgcctctcctgatgtccttcacagatatattctttacattaaagcccacattgtctccaggcaatgcctcttgcaaggc
ctcatgatgcatttccacagacttaacttctgtagtaacattactaggtgcaaaagtcacaaccatgccaggcttcaagacaccagtctccactctgcca
acaggcacagtgccaattccaccaatcttgtacacatcccggcaaaggagccttanaagccttgccaatggggtcgctgaaggaggaataaatgccatca
agtgcttctagcagatggcaaaccacgggcatt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: