XB-CLONE-4352497
Clone Name: IMAGE:7027419
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Gene:
psmb2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784212
[+]
taattaatccccccccccccatggagtatctgattggcattcagggcaatgacttcgtacttgtggctgcggacacagtgtgtgccaacagcatcattaa
gatgaagcacgatgtggacaagatgtttaagatgagcgagaagatcctgctgctgtgtgtaggagaggcaggtgacacagtccagtttgcagagtacatt
caaaagaacgtgcagttgtacaaaatgaggaacggctatgagttgtctcctactgcagccgcaaacttcacacggagaaatctggctgactatcttcgca
gcaggaccccttaccatgtcaatctgctgcttgctgggtatgatgaacatgacggaccctcgttgtattacatggattacctagcagctctagcaaaaac
gcgttttgctgcacatggctacggggcttacctaaccctcagtattctggatcgctactacaaaccagacctgacaagagaggacgcagtagaactacta
aagaaatgtatagcagagctccagaagcgctttatcctgagcctgccttccttcacagtgagggtcatcgataaggatgggatccatgacttggagacga
ttcctgcttcaggcctttaacctttacctctccctgactcgcctcctcctttttctttttaaatattaagggttaatttgtaccaccggtatgaaacaaa
taaattaccctgtgcatttgcatggggggactccccgggaaaaaaaaaaaaaaaaaggatccccttagtttctagagacg
3' EST: CF784211
[+]tttttttttttttttcctgggagtcccacatgcaaatgcacagggtaatttatttgtttcatacagtggtacaaattaaccttaatatataaaaagaaaa
aggaggaggcgagtcagggagaggtaaggttaaaggcctgaagcaggaatcgtctccaagtcatggatcccatccttatcgatgaccctcactgtgaagg
aaggcaggctcaggataaagcgcttctggagctctgctatacatttctttagtagttctactgcgtcctctcttgtcaggtctggtttgtagtagcgatc
cagaatactgagggttaggtaagccccgtagccatgtgcagcaaaacgcgtttttgctagagctgctaggtaatccatgtaatacaacgagggtccgtca
tgttcatcatacccagcaagcagcagattgacatggtaaggggtcctgctgcgaagatagtcagccagatttctccgtgtgaagtttgcggctgcagtag
gagacaactcatagccgttcctcattttgtacaactgcacgttcttttgaatgtactctgcaaactggactgtgtcacctgcctctcctacacacagcag
caggatcttctcgctcatcttaaacatcttgtccacatcgtgcttcatcttaatgatgctgttggcacacactgtgtccgcacccacaaatacgaagtca
ttgcccctgaatgccaatcagatactccatggggggggggggggattaatttaactcgaggtgcaaaaagaaacaagtaactttgtattcggggcccgga
ataaaacccgaaggtgcaattt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: