XB-CLONE-4352551
Clone Name: IMAGE:7027442
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Gene:
anxa4
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784177 [+]
taattaatcccccccccccccccgtctccctgcccagccctgtttggatccgtcgactcagctgacatctgcttggagagtctctgctttgcagttggtt
gatatcatggcaacactaggaactaagggtacaattaaaccctatgccaatttcaatgcagccgatgatgtgcagaagctgaggaaagccatgaaaggag
caggcaccgatgaagatgctatcattgatgtcattgcaaatagaaccctttcccagcgccaggaaatcaagactgcttataaaaccactattggaaaaga
tctggaggatgatttaaaatcagaactgacagggaactttgaaaaagtcattgtgggccttataacgccatcgactctctatgatgtggaggaactgaag
aaagccatgaagggtgcagggacagatgagggctgtttgattgagattctggcatctcgcagtgcagaggagatcaagaacataaatatcacctacagaa
taaaatatggtaaatctctggaggacgatatttgctcaaacacatcttttatgttccaaagagttctggtatctttggctgctggtgggagagaccagaa
cacccatgtgaatgaagcactttgctaaacaggatgcccatggaactatatgaaaccgggggaaaaaaaatggggggactgatgaaggtgaaattctcaa
ctatccctttgcaccaagaaacgggaaacaacttggttgaaggtttttgaatgaataaaaaaaaagattggcaaaaaaaaatacttcgagcccagtaata
aaaacccgaaaag
3' EST: CF784178 [+]tttttttttttttttaaaaagggataagaaggtttttatattgcgtttaagaagacatgacagattgatttcttggatacacagaaaggttacgttggtt
agtcttcccctccacaaagtttcaagagcaccttcctataatctccagagcagtcacccttaataaaggaatgcagagatttgccatacatcttcttgaa
ctcgctgcggatttccagcatgtcaatttcacagcgagaaaccataactctgatcagagttttatcatctgtccccagacccttcatagatttgtacaac
cgttctgcaaaatagcctggcctactcctcaagcatttcacgactgccaaaagagcatcttcaaagtgtccagacatttcggattttatactggcctcga
gatctttttttgcaatctttttatattcatcaaaaaccttcagcaagtggttccggtttctgctgcaaaggatagttagaaatttcacctcatcagtccc
ccacttcttctcaccggcttcatatagttcattggcatcctgtttaacaagtgcttcattcacattggtgctctggtctctcccaccagcagccaaagat
accagaactctctgggaaaataaaagatgtgtctggagcaaatatccgtcctcccagaaatttaccagaattttattccgggagggggatatttatggtt
cctggatctcctccggccctggcaaaatgccaaaaattctaaatcaaagaaggccctctttttggtcctggagccctttgaggggtgtttctttaggttc
tctccccttcattaaaaaggtgcgaggggggtgtttaagggcgcctcatggn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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