XB-CLONE-4352822
Clone Name: IMAGE:7027548
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Gene:
lrpprc
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784005 [+]
taattaatccccccccccccctctgtccacctggagaagggagacttaccagccgccatggaaacactatttacctgcagcaagaagtacaactgcatgc
ctcgcttgcatgatgtcctctgtagattggtggataaaggggatactgatctgctgcaaaaagcaatggaccatataagccaggagaggggagagatggc
aatgctctatgacctgctctttgcgtttcttcacactggaaagtaccaagaagccaggaagatcatcgagactccagggttgagagctcgtcctggaatg
ttgcggtggtttgctgagaaatgcattgcaacacatcagatggaagctttggaaaatttggtagatatgacccagacgctgtttgattgcgacagagatg
acctgtactactatcttccaaagctcggcaaggaaaatgacgaccgggaaaaaagcatgattctggcgtgggactaaaatttcaataaaaagaatgtgat
ccccctaaaggaggagaatacattggaggcctgggtggtcagaatattttttctacggaataaattgccaccaaaaaccttctttttttttgatggcacc
cattatcaatctttggtaaccaaaagtataggctttgctccgaaatccaataaaacctcccctctacgcatatctccttaccctgattgggcctgctccc
ctaattccccccccgaaaaatccactctccctttcccactaagataacggccctttcaaaggtttcccgcccgccccataatagtaaagaggaaggcgca
cagtaaaatgtccaataaataaaagtacgtttcttttgggaagggcctcaaaaaaataacccccgtctctttttttgcggaacgctgcggtttttgcggg
agaaattcacagacggctactaccttcagttaaactgccgctttgctcaaatagggacatgcccctgaaagaaaattcccccaatagacgttttattgac
a
3' EST: CF784004 [+]tttttttttttttttatttttttgttttttgttttattccccacccacattaaatatcccttgcaggtcagatttataacacttcccagcagcaacaaaa
tacatataaaaaaaaaataatttgtaagtgttacagaaattaaaaaaaacgagagctaatgttcatcatcgtaggagtctgccttgtctttctttaattt
gtctgcgtagaatttaaagctttcagggaggctcgctgaaagggaccggctctcccgcttccctcagaaggactgccaagcgtttcaggaacagctcgtc
cggactcacactttcgcttttcatcttctcgtataaagcggtcgctgcagtcgcatctttgtctgtcgcgtaacatttcataaggtaggagtaggctgtt
tccctctctgaagaattcagggatcagttctagcagatctttgaatcagctgcccctggcctggtatctgtgcagaatcttgagatgaaagatactagga
caggggtttgcttcggctatagaaccgcacctctgcaagaaaaaaacgggccatcgtccttttcttccaactttggaatgtacttgcagggaaaaaggtc
ctggtaacgggttctgttcacagcaaaactggattgggccaatcttcttccgccatggccacctcaattttcttctagggcccaacttccaacctttttc
tttccattggcccttcccgggaacccattaggaaaaataactggtggaccctggggaaacccggtttcctggtaaaaaaaaggagccatctaataagacc
ccgaattggcccttctttccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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