XB-CLONE-4352864
Clone Name: IMAGE:7026328
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Gene:
eef2.1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783979
[+]
taattaatcccccccccccccgctcttccgcctgtttgtctgctgtgaggcagactgtccgtatcggcctaagcccgtcccgtgaagagaatccaccgcc
atctgccacaatggtgaacttcacggtagaccagattcgttccatcatggacaagaaggcgaacattcgtaatatgtcggtcattgcccatgtggaccac
ggtaaatctactctgactgactcactggtatgcaaggctggtatcattgcttctgctagggctggtgagactcgttttacagataccagaaaagatgaac
aagagagatgtatcaccatcaaatcaacagcaatctctctcttctatgagctctctgagaacgacttggcttttatcaagcaaagcaaagatgggtgtgg
cttccttataaacttgattgactcccctggccatgtagacttttcctctgaagtaactgctgctcttcgtgtcacagatggtgccttagttgtagtggac
tgtgtttcaggcgtctgtgtgcagactgaaaccgtgcttcgtcaagccattgcagagcgcatccgcccctgtgttgatgatgaacaaaatggaattgtgc
tctttttggagctgcagttggaaaccagaggaacttctaccaaactttttcagcgcattgtgtggaaaaagttaaacggtgatcattttcccacctaacc
ggtgaaaggaataaacctggccccctctggggtaattattcatggatttgaacccccgggttcctttgggcaaaaatttggggtttccgggcctcctggg
tcttggacatgggcgcttggggcccttctttttattatttaaaataccaccttttgtcccaaaagaatatgtttatgggtccccccataaaaaattt
3' EST: CF783978
[+]tttttttttttttttcaattaaatttttttaatggcaccaataagtcattcaggttattgcagtacatctttgctctacattctgccctcgttcctgtca
accctcaaattcacatttacttccccagccatccaagatgttttcagagagcagaagctacccatcaagaataaatagaaagttatgatcatgccaatga
aagattatattcagggttccaacagaatgttgcagggaatgctgacatcttttgggtatagttcatttccacttaatcaatgctgacaggagtaaagcaa
aggtttacagcttgtcaaggaagttatccaaggcttgaacaccctctttcaagcccttcctcttccgggtctctgccaccacttggaaaggcctggtggt
gttatcgaaaggatctccaggtagaatttgccagtggtcaaacacacactgagggaaggcctggccaccagtgtttgatcttaagtcagcagtgaaacca
aatgactcatttacaggcaggtatgccttaaccacaaacattggtgttccagccacctgggattcttcaaagacatgcccacgctttctgttcaagacac
catagatacctcctaccacctgctcagggcactggatttcaacaagtagattgggtccatcagacgtggctgagcagtcaacacacaagcatacaagacg
cggcgtgcaattggaatgatctgtcctcctcctctatggatggcatcagcatgaagtgttacatcctggacatcaaaacgaaca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: