XB-CLONE-4352873
Clone Name: IMAGE:7026332
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Gene:
unc45b
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080138 [+]
CCTTAGTTTGGCACAGACAACTCTAGTCAAAGCAGGTCTAGGCTTTAGTGTGTGCTCAAGTTAAACCCCTTTTCTTCTCTGCTTGTGTGCCATTCCAGAG
GACTGGACCATTTCATTGAGATCTATAAATAACTGAGAGGGAGGCTTCATGCCAAGAGACTGCTAAGGAACAGAAGTTTGATTTCAGAGAAGAGGGGACA
CAGAAGAAAAGAAAGGAGTTCATACTTCCAACTATAAGATGGAAGACCCAGTGCAGCTAAAGGAAGAGGGGAATAAGTATTTCCAATCTAATGAATATGG
CCAAGCTATTCAGTGTTACAGCAAAGCCTTGAAGCTTATAACTGATAAGAAGATGCAGGCTGTACTCTATCGGAACAGGTCTGCTTGTTACCTAAAGCAG
GATAACTATGTACAAGCAGCTGCAGATGCATCAAAAGCTATCGATGTGGATGCATCAGACATTAAAGCCCTTTTCAGGCGCTGCCAGGCTTTAGAGAAAC
TGGGCAAGCTGGACCAGGCCTATAAAGATGTTCAGAGATGTGCAACCCTTGAGCCAAAAAACCGAACATTCTTGGAGACGTTACACCGACTAGGCACAAA
TATCCAAGAAAAGCTACATGTTCAGTTCTCCACGGACTCCCGGGTTCATAAAATGTTCGAGATCCTATTAGATAAGAACAGTGAAAAAGAAAAGAGAGAG
AAGGCAGCAAACAACTTGATAGTTTTGGGCCGAGAGGATGCAGGAGCTGAACAGATCTTTCAGAACAATGGTGTAAATCTTCTTATGCAGCTAATTGAAT
CGAAGGATCCAGAAATGATCCTCTCAGCAATCAGAACTCTCTCTGGCATGTGCACTGGGCACAGAGCACGGGCAACGGCTATTGTGCACCTTGTGGGTAT
CAATAAGATCTGCAGTATCATGGCAGTAGACAATGAAGAAATTGCTCTAGCAGCATGTAACCTGTTGCAAAACATTGTGGACTCTTTAACCGGAGAGGAT
AAGAAAGCACATGGGAAACAGGAAGCTCTTGTACTGGATACCAAAAAGGATCTGAAGATAATTACCACTCATTTGTTGGATATGCTGGTTAGTAAGAAGG
TATCTGGTCACGGCAGGGACCAGGCACTTAATCTGCTGAATAAAAACATCCCACGTTATGATCTTAAGAATAAAGATAACTCCAAGTCTCTCTTTGTGGT
GGATGCAGGGCTGAAAAAAATATTAAAGGTTTTAGGACAGGTCCCGGAGCTGCCAAATTGCCTCCCCTTGACACCAAACACACGTTTAAATGCCTCAGTT
CTGGTGAATAAACTTTACGATGATTTGCGCTGTGACCCAGAAAGAGACAATTTCAGGATAATCTGTGAAGAATATATCACTGGCAGTTTTGACCCTAAGG
ATATGGAAAAGAATCTGCATGCCATACAGACAGTTTCTGGCATCTTGCAAGGGCCATTTGATCTTGGCAACAAACTCCTTAGCTTACAGGGGGTGATGGA
AATGATGGTTGCTTTGACTGGATCAGAGAATGAGGTCGATCAGCTGGTGGCTGTGGAAGCTCTGATTCATGCATCTACAAAGCTTAGTCGGGCATCCTTT
ATTATTACAAATGGAGTGTCTTTATTAAAAGATATATACAAGAAAACCAAGAATGAGAAAATAAAGATTCGTGCACTTGTGGGTCTGTGTAAACTAGGAT
CTGCTGGTGGCACAGATTATGCTTTAAGGCAATTTGCAGAGGGATCTACTGATAAACTAGCAAAGCAATGTAGAAAGTGGCTGTGTAACCCCAGTCTCGA
CATACAGACCAGGAAATGGGCAGTGGAGGGCTTGGCTTATCTGACACTTGATGCTGACGTAAAAGATGAATTTGTAGAAGATGAGCAATCATTGAAGGCC
ATGTTTGAGTTATCCAAGACAAGTGATAAGACCATCCTTTACTCTGTGGCTACCACACTGGTAAACTGCACAAACAGCTACGATGTTAAAGAAGTCATTC
CTGAAATGGTGCAGCTGGCAAAATTCTCCAAGCAGCATGTTCCAGAGCAACATCCTAAGGATAAAAAGGACTTTGTGGAGAAACGTGTCAAGAGGTTGCT
AAAGGCAGATGTTATATCTGCTCTATCCTGCATGGTGAAAGCTGATAACTCCATTCTTACAGACCAAACCAAAGAACAATTATCAAGAGTTTTCCTGGCA
CTCTGCGAAGACCCTAAGGATCGAGGTATCATTGTTGCACAAGGAGGAGGCAAGGCTATGATCCCTCTTGCACTGGAAGGAACAGATGTTGGCAAAATAA
AGGCTTCTCATGGGCTGGCTAAAATTGCAGCTGTATCAAATCCTGATATTGCATTTCCTGGAGAGAGGGTGTATGAAGTGGTGCGTCCGCTTGTCAGTCT
ATTAAATACAGAACGGGATGGAATTCAAAACTTTGAAGCCCTCCTTGCTTTGACCAATCTATCTGGAAAGAACGACAAATTGAGACAAAAAAAAAAAAAA
AA
5' EST: CF783973 [+]taattaatcccccccccccccccttagtttggcacagacaactctagtcaaagcaggtctaggctttagtgtgtgctcaagttaaaccccttttcttctc
tgcttgtgtgccattccagaggactggaccatttcattgagatctataaataactgagagggaggcttcatgccaagagactgctaaggaacagaagttt
gatttcagagaagaggggacacagaagaaaagaaaggagttcatacttccaactataagatggaagacccagtgcagctaaaggaagaggggaataagta
tttccaatctaatgaatatggccaagctattcagtgttacagcaaagccttgaagcttataactgataagaagatgcaggctgtactctatcggaacagg
tctgcttgttacctaaagcaggataactatgtacaagcagctgcagatgcatcaaaagctatcgatgtggatgcatcagacattaaagcccttttcaggc
gctgccaggctttagagaaactgggcaagctggaccaggcctataaagatgttcagagatgtgcaacccttgagccaaaaaaccgaacattcttggagac
gttacaccgactaggcacaaatatccaagaaaagctacatgttcagttctccacggactcccgggttcataaaatgttcgagatcctattagataagaac
agtgaaaaagaaaagagagagaaggcagcaaacaacttgaatagttttggggcccagaggatgcanggagctgaacagatcttttcagaaacaatgggtg
taaatctttcctaatgcagcttaa
3' EST: CF783972 [+]tttttttttttttttgtctcatttgtcgttctttccagatagattggtcaaagcaaggagggcttcaaagttttgaattccatcccgttctgtatttaat
agactgacaagcggacgcaccacttcatacaccctctctccaggaaatgcaatatcaggatttgatacagctgcaattttagccagcccatgagaagcct
ttattttgccaacatctgttccttccagtgcaagagggatcatagccttgcctcctccttgtgcaacaatgatacctcgatccttagggtcttcgcagag
tgccaggaaaactcttgataattgttctttggtttggtctgtaagaatggagttatcagctttcaccatgcaggatagagcagatataacatctgccttt
agcaacctcttgacacgtttctccacaaagtcctttttatccttaggatgttgctctggaacatgctgcttggagaattttgccagctgcaccatttcag
gaatgacttctttaaacatcgtagctgtttgtgcagttttacagtgtggtagccacagagtaaaggatggtcttatcacttgtcttggataactcaaaca
tggccttcaatgattgctcatcttctacaattcatcttttacgtcagcatcaagtgtcagataagccaagccctccactgcccattttcctggtctgtat
gtcgagactgggggttacacagccacttttctacattgccttggctagtttatcagtagatcccccctgcaaattgcccttaaagcataaatctgtgcca
accaggca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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