XB-CLONE-4352878
Clone Name: IMAGE:7026334
|
Gene:
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF783970
[+]
taattaatcccccccccccccggcagctcctttggaaccatcacaagatgttggggctctgggggctgtttttcttcctgctgggatgtgtgcaagggga
gccggtctgttacgacaagatcgggtgtttcagcgacgagaaaccatggagcggaacgttagagcggccaatagcgaggctgcccagctccccggaacat
atcaacactcgcctcttattgttcactagagaaaatgctgaaaacttccaagagctccgccccttgaacccctcggcagtgtcactgacaaacttcaaga
ccagcaggaagagtcgatttattatccacggattcatcgatgagggacatgagaactggctggcgaatacgtgcaaagcgatgttgacagccgaagatgt
gaactgtttctgcaccgactggagtggggggtcccgcattatatacattcaggccgccgataaacatcctttgctggcgggggcaagatcttggccaact
ctccgtggcttacctcccctagcacggattcaacaaccctctccgtccaaacgccgccacgtttatccggtcccctcccccttgatgctctacacaacat
caccgggttcaccggttggttaaggcgggatttccctgggacatttgggcccaaaatttcccgtgggcctagcaatccgtggttgggggcccgctaactt
tcccaaaagacatccccccaattttaggcggttataaacccggccattttattatcgacacggccctctattttagaaaaaatggcccttattctttttt
cacaccccgtgctcccccgcttattcttcccacacatgtgggctatatgggccgttcgagacacacttttgtagttctcaccttgcccatgatcggtttt
ctcattcccactttaactgttgttctccccacccacacatctgcgtcttctcgccgtccccccccc
3' EST: CF783969
[+]agaaattcacgctgccggtggtcttcacggtgaccctgtatctccagcgtgaataaggcttttcacttccagtgtttaagaaataagaatagtcgcttgt
tccatggctggaaaaggcgtcggcatagtggcccattaatgggcaaccagttgtgggacatgggaatccggtaccctttgtaaatgcttcatatgaagtg
ctggggtatccaacgaatccatcgggagacgagatactttcagtgtaatatttgtagcttctaaggtggttacaggcaaatatatccttggatccttccc
agagtccatcgatgtctaagagcttggccaggatgggtttgctgcaccccggcatgttctcgcccccgttagggaagaaatccatgtgtgcgactgactg
gctcatcccataacccatttttggggatcagcggngtcggtatctgtatgaatggcatctacaaagacggcatcggttggatccagtctgacctcgatgg
ggggtgttttggaaatacgggccaacccggatcaagcccccggtattcccccccatgccgggcatcccgctttgccaccttcccccgggaagtgttgaaa
accccaaactgtgggcccaaaaatggtgcacctttggacaaaaaagggaaatttcaatccttggccaaaaaaagggtaaccccaattaaaaata
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: