XB-CLONE-4352890
Clone Name: IMAGE:7026339
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Gene:
prss8
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783963 [+]
taattaatcccccccccccccacactcacgattgcatccacctgttcacatctctccggcaccgacaggggccacttttatccacacttacccctctggc
acatcgggctgcctgtggctttaaagtaaagggcaagtacgccgtaagggagaaggatggagcatctcccacttctctcgctgctcctgttagcagtttc
ccattttgagctgggcagatctcaggagggtgtacagagccgcattgtgggcggccatgacgccagtgagggaatgttcccctggcaggccagcctgagg
tacgatggcaatcatgtgtgcggcgcagccctcatcagcgcaaacttcattctgacagctgctcactgcttcccctcggaccactcactcgttggttact
ccgtgtaccttggggtcctccacaggaagaggcggggacctcaaatttcctaacattccgaaaacttaagccaaagtgaattaatttatcccctccttaa
tcccctccaaaactttccctttcgggggacctggaaaataacacatgccccctgaaattacccccccgaaatatcttttttaaccatgctgggacccaaa
accccattctttctctttacaccaaaccggcctccaaagtgtgttaacttttcctctcaacttgggacaatggacactgtgtccacacgcgcattttggc
ccgcggcgtccaaaataacccacaacacagccggagttaataatccctcacggggcgagtccccaattaaatacacgcctaaggattgtgttataaatca
tcttaacataccatttgtaccagccgcttccaaatacaaataaccatgcactaagttaacccactacccataaccaaccgattcccgttcgccacctttc
cagttgcgcttttccaacccccccacgccgatgatgacgcattttcacccaactcgcaggcttccn
3' EST: CF783962 [+]ttttttttttttttttttttttttttttttttttttccgttttttttttttttttttttttttttttttttttttttttttttttttttttttttttttt
tttttttttttgggaaaaaaaaaaacccccccggtttttgggggggcccccaaaaaaatttggaaaaaaaaaaaaatttggggggaacccccaaatgggg
aaaaccccggggaaaaaaacccccaaaggggcccccccccccccccccattgggaagggggccccccccccccaaaaaaaaaggcccccccccgggggcc
aaggggaaccctttccccccctttaaaaacccccccgaaaaaaaaggggaaaacccaaaaaagggcccccggcccccgccccaaaaaaaaagggggcccc
ctggggggggggaaaaaaaagggggtggggcccaaaaaaaccccttgttttcccggggtccaaaaaaaagccccccgcaaaaaacgggaaaaaccccctg
gggggaaagccccgaaacccccggggggggggggaaaaaaagaaggaaaccccccgccggttttgggggccgccatttcccccccactaaaaaccggggg
ccaggaaagcgctttccccaaccccccaggggggagggggccccccacaaaatctccggggggggggggtattcccccccccgcgcaaaaccggggaaga
aaaaattttcccgggggggagggaaacaaaaaaaaaccccccccaaagaggtttattttttctaccccgccacattttgcctctctcgcccgcgaaaaaa
aaaccatcccgtgttctaacacacatgaggggagtttttttgcgcgccccctggaaaaaaaaaaaaacatcctggtggggggt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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