XB-CLONE-4352904
Clone Name: IMAGE:7026344
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Gene:
vdac1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783954
[+]
taattaatccccccccccgtccccttgcactgtcactcagctctccagcagcagcagcagcaggcagcctctcagctaggaagaaaatggccatacctcc
ggcatatgctgatttaggaaagtctgcccgagacatcttcactaaaggatatggatttgggttcattaaacttgatttgaaaacgaaatcagaaaacgga
ctggaatttacaagctcaggatcagcgaatgcagagactaacaaagttgctggcagcttggaaaccaaatacaaatgggcagaatatggccttactttca
cagaaaagtggaatacagataacacattagggacagagatcactgtagaagaccagcttgcaaagggtctgaagctgacatttgactcttcattttctcc
taacacaggaaagaagaatgcaaaagtcaagactgcatacaagcgagagcaccttaacattggctgtgacatggatttcgatattgctggcccatcagtg
cggggagctgcagtgtttggctatgagggatggttagctggctaccaaatgactttcgaatcctcanagtccagagtctcccanagcaactttgcagttg
gatacaaaactgatgaatttcagttgcataccaatgtaaatgacggtacagagtttggtgggtctgtatatcagaaagttaatgataaactggaaactgc
aatcaaccttgcatggacagcaggaaaacagcatacacgcttcggaatagcagccaaatatcaaatcgatagtgatgctttcgttttcagctaaagtgaa
ttattcaagtcctattggttttaagatacactcagacttctaaaccctggtattcaa
3' EST: CF783953
[+]tttttttttttttttcctttttttttttttttttttttaaacttgtactcggactttattacaggtgatgttgtgaacagtatcagggatctacagaatg
gaagacactacattttctaaaaaatgaaaataaaactggggtagggtaaaaaccaatcaaacatgtaagtatgtgtataaagaataaaagattcttccct
tgcatatggctctgtcaagctgtccagtactctttatgggaagggaatcaagtattgtcactggctcgttctttgtgtgcaccgataatatgggatatct
tcttatgggctacagatatttataataaacacatttttaataagacttttaacagtgtatgaaatagttcactttaagtccctgctttgttcaagtgcta
aatatacacgttgtgcatatgacaaaactctgatcctgcacccgtgatgttgatgcaggaccgtaacttcctagttccgaacaatgaattcttactaaag
gaattgtttgtttcatctaaaagtccgttagtagtgtcaataaattctgctctataggttattaaaaaaaaggcctttaatactctgttgtttagcaaca
cgtgtataaataagcagctatgaagtatttccaggctggcctggatttgtaaaatctgtaccctagagtttctctcaccatttctcacagcagcaataac
tctgtcacacacacttgtggtttggtttttccaggggaatcaatagtaaatcgaacataggtggggttgttttggcatccccggaaaccggaaactttac
aagggtggcacttaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: