XB-CLONE-4352925
Clone Name: IMAGE:7026351
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Gene:
rpl10
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783940
[+]
taattaatcccccccccccccgctttctggtgttggcgaggtgcagccgccatcatgggccgccgacctgcccgctggtgagttgcgcttccctggggag
ctggacggttaccaagcgtatgaggcctgggtgtcccgcctgtcacgtgtatggcttccctgtctcatcgtcccctggacgggtctcacacctacctatc
caatcccctcctcactgtatccgtcctcccccattaccgcccccacttcccccggcgtatctgtcacattgcggtacccgtttctgccctcctagccgcg
catgttgcccagctatccccgccttgtcgctcgttcccgctctccggtatttttctgccctgccttaccttcactcggattcccatgcgtcctgcgtttt
ttgggtatatccttgtcgttattccccctttaccgaccccttcctccgactaacttctcccaacatcggtcatttcacttgctgtattacttccgagact
cgttcgtgcggaccccatttttttatgccccctcctaccactttttttcttatttaccccccattttccgagtatttttgctcatattccccaagcctat
acctcgccgccctccacgaataatggtttttcgcttccgccctcgttgacaattctatatctttcccctcgcctctatatcgcgtttaactacntcttgt
cgccacgaaccgcgaattaatagagtccaccctcagacacccactctttaccaccgcccccctcgttttttattatcacccattagcgcacccatccctt
acatcaagatttgcgtttgttttttctccacctccccctccccccgtgctttttattatcagccctctattccgataatttattatcatataatagaaaa
ccgcgtgtctatagttgcgcgcggacctaagagtttacgtaattatcccgttgtttagtgagaaaaaacctcgcttgagagccccaccattatacccata
taatcatagtctntatttcgccacatatatagattcttttgtattatatccaccttttatatatgtccccacgcatgtcttcggtatatactccccttct
ctcacccaagagaaacaaaattncacaccccccacccgcaccctatttaccccacatagatccccagttaccaccactttaattgtaaatgatttgcccc
cccctcctttccggctatgacctatttgcaccccctcgcctcctcacgtgtcatttttattgcccgcattaccccccgggtatatagctctatatatcgc
3' EST: CF783939
[+]tttttttttttttttcgacatccaataggttttattgggagagaggctctgctagaagtagaatctaaacagcatgaattgctctccatttgtccagggg
gcccctgttgggaatgtacttcaccccacagccgtcagggatcagccgcttctccgcaagcagagactcaaactcgtcagcgttgaacttggtaaaaccc
catttcttagaaatgtgaatcttctgacgcccagggaacttgaacttggctcggcgtaaagcttcaatcacatgctccttattttgggtcttggtgcgga
tagacatgatcacttgaccaatgtgcacacgtgccactgtgccctggggtttgccaaaagcaccacgcattccggtctgaagtctgtcagcaccggcaca
tgataacatcttgttgattctgatgacatggaaaggatgcaggcgaacccggatgtggaagccatccttgccgcagctcttcaccatgtacttgttggca
cagatacgggcagcttccagagcttctgaagagagctgttcatattcatcagataccatgtgaccacacaatgggaactcatccacctttgctttcttgc
gccccaagtcaaagatgcggattttgggatctgggactcctctacagaaacgggatttggggtacggcttgttcttgcagtatctgtaactaaacagtgc
aggaaagagttaatgtagagcgtaccantgaaggaatggggggggggggggatttaactggtttaagcaaggttaatcccaaatgtgttgccgggccggt
a
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: