XB-CLONE-4352936
Clone Name: IMAGE:7026355
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Gene:
urod
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783933
[+]
taattaatccccccccccccccgaagagctcaaactatatgacagagatgagtatggagcagccagaacacttgctgcctgaatgcttccctcctctcca
taatgacacatttctccgtgctgctcgtggtgaggagtgcaaccatgttccagtgtggtgtatgaggcaggcagggcggtatctgccagagtttcgtaag
acacgcgctggacaggatttctttgcgtcatgtcggagccctgaaatctgctgtgaactcacactgcagcccctgcggaggtttcctcttgatgctgcta
tcattttctcagacatccttgtgataccacaggctctgggaatggagatccggatggaacctgggaggggacctgtattccctgaacctttacagacccc
acaggacctgcacaagcttataagctcagtggatgtatcagaggagctgggatatgtatttcgtgccatcacacttacacggcataaaatagaaggaaag
gtgcccctgattggatttactggtgcaccatggactttgatgacctacatgatagagggaggaggatctaacactatgtcccaaacaaatcgctggctgt
accaataccccctctgttagccacccagcttcttgaagcacctcactgactgtcattgggggaatatttgagcggggccaaggtaaccacctgggttgcc
tccatgccccttgcaaagtggtttggacctccctatggcctgggctgccccttgggacacccccccgaagcttttccggaatctctttcccccttccccg
ttacactttcagcactatgatattttccca
3' EST: CF783932
[+]tatctgggtacaaaccatggcccagatttgcaatgtacccacgctggccaaagccttctatcatctttttcacagttttttcaatatcctccttgctggc
atacaaagcacagggatccatattgccctgaagagtcactcgtttgcccgtgcgttcccgagcttcctgtggtcttatggtccagtccaatcctaccacc
tcatagccagactgggacagatcctccagtgcatagtgggcatctttagcaaagacaatcatgggcacctgatccaatccctcagctttcagtttatgtt
ttactctgcgggaaatatctctaagatacgggagagaaaaatccgagaactgctggggtccaaggcagccagcatgggactcaaacacttgcaaggcctg
agcacccgctgctacctggcccaccaaatattccacaatgacatcagtgagttgcttcagaagctggtggctagcaaaggggtgttggtacagccagcgc
tttgctttggacatagtgttagatcctcctccctctatcatgtaggtcatcaaagtccatggggcaccagtaaatccaatcaggggcacctttccttcca
ttttatgccgtgtaagtgtgatggcccgaaatacatatcccagcctcctctgaaacatcccctgaactttctaaatttgtgcaggtcccttggggggtct
ggtaaaggttcagggaaataaaaggtccccctcccaggtttccatcccgggatcccccatttcccaaaaaccctgttggtaatcaaataaggcaggcccg
gaataataatttaataaccaagcaccccaagagagacaacaccccccccccaagggtgtctatgtaaaatgtggcgaggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: