XB-CLONE-4352946
Clone Name: IMAGE:7026359
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Gene:
myl11
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783927 [+]
taattaatccccccccccccccccatgtcactctctccttctgatcaggacaagcaccaagctcaaccgaggacatggcaccagcaaagaaggccaagag
aaaggcagcagaaggtggatcttccaatgtgttgtccatgtttgaccaaacccagatccaggagttcaaggaggctttcacagtgattgatcagaacata
gacggcttcattgacaaggaagacctgagggacacattcgcagctatgggccgcctgaacgtgaagaatgaagagttggaagagatggtcaaagaggcat
ttggccccatcaacttcaccgtcttcccgaccatgttcggcgagaagctcatgggtgctgatcccgaagacgttatcactggggcattcaaggtgcttga
tcctgaggccaaaggatccatcacgaagcatctccttgaggaacccctaaccacataacgtgacagattcaccccagaggagatcaccaatatgtggaca
gcgtttccccctgacgtcgccggcaacgtaaaccacaagaacatccgctatgttaccccccacgtaaaggacccagacctcctaaaaaacgggcccagcc
cttgcccttttggaaggacgaggggtttcccccccctgttagcccccgacccttggccgtatgtggcgctcttactgtaaaacacttcctcttcgttcac
agtccacaactggaagagttcttccccttctatacgcttgccctagataagccccctccctttccaccggccgtttccttgatccccctccgcccctagc
ggagtccttgtcgcccccccgccggttttttctcccccttatcccgccgcattcttttattcc
3' EST: CF783926 [+]ttttttttttttttctttttttttgttttgaataaaaagttgactttattgttcttcccttgagggctccagagttccagtttaagacatcaaagagcga
aatgtgagggagggtaaaagaggagggaaagggacagcgagaaaggggacaagaacaacagagaggggtcccctttcagaccacccctagaagaaataaa
ttacagttgtgtctctgatgggatgagtttataaaataagacgcaatatacagcaaggttggtgctgggcgtgatggcgcaccccctccatcaggagggc
aagactgggcctgtttattcctggtctttgtcctctccgtgggtgatgacatagcagatgttcttgtagtctacgttgccagcaacatcaggggggaacg
ctgtccacatgttcttgatctcctctggggtgaatctgtcacactgtgtggtaaggagttcctcaaggaactgcttcttgatggatcctttggcctcagg
atcaagcaccttgaatgccccagtgataacgtcttcgggatcagcacccttgagcttctcgccgaacatggtcaggaagacggtgaagttgatggggcca
gatgcctctttgaccatctcttccaactcttcattcttcacgttcaggcggcccatagctgcgaatgtgtccctcaagtcttccttgtcaatgatgccgt
ctctgttctgatcaatcactgtgaaagcctccttgaactcctggatctgggtttggtcaaacatggacaacacattggaaagatccaccttctgctgccc
t
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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