XB-CLONE-4352949
Clone Name: IMAGE:7026360
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Gene:
ddb1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783925
[+]
taattaatcccccccccccccgggtcgcaaggtttccggtccccattggtccgcggccttttttgctgtcttcattcttttcccgcctgtcgcttctggg
atcgttgcgacaggcactcacttcataggaaccatgtcgtacaactacgtggtgacagctcagaaacccacagcggtcaatgcctgtgtgacagggcact
tcacatctgaagatgacctgaatcttctgatagcaaaaaatacccggctagagatctatgtggtaacaccagaaggtctgaggccagtcaaggaggttgg
aatgtatggcaaaattgcagtgatggagctcttcaggcctaagggtgagagtaaagacttgctatttatactgactgctaaatacaatgcatgtattttg
gagtacaaacagaacggggacagcattgatatcataactcgtgcacatggcaacgtacaggatcgcattggccgcccttctgaaactggcatcattggga
tcattgacccggactgcagaatgattggtcttcgtctttatgatgggctgttcaaagtgataccattagagcgggacaataaagagttgaaggcctttaa
cattcgcttggaggagctgcatgtcattgatgtgaagttcctgtatagttgtcaggcccccactatttgctttgtgtatcagggaccccaaggcagacat
gttaagacgtatgaagtttctctgagggagaaggagttcagcaagggatcctggaaacaagaaaatgtagaggcagaagcatctattggtcattgcagtt
tccgagccttttcgggggtgccattatcattggccaagagtccattacctaccacaatggaaaacaaatacttagccaataccccctccccttatacaa
3' EST: CF783924
[+]attccatttcggataatccttaaagaaccctccttaaaggctccagagcaggtcactaactgcccctgtccttgcctctccagatccacaacacacatgt
ctacaatggggcccaagtttgtgaaagtttccataacaacaacgtatgatccctgttcgttgctctctgctgtaagcttcaccagctgggaatctccgag
acgagagcccacaaacacaactccattgtctaaataagtcagacattcagcaatggaagtctctcctagtaattccacacgcagatccttcagtgtgaca
cttccatccatctgctcctccttctccaataaaagcataaacaggcggccctccatgtcacccagcagataccgcgaaccattgacatctactcggttat
ggcacacaatggtgctttgcttgataatgggaggtgctattgctaggtacttgtctccattgtggtaggtaatggactcttggccaatgataatggcacc
cccgaaaggctctggaactgcaatgaccatagatgcttctgcctctacattttcttgtttccagngtcccttgctgaactccttctccctcagagaaact
tcatacgtcttaacatgtctgccttgggggtcctgatacacaaagcaaatagtgggggcctgacaactatacaggaacttcacatcaatgacatgcagct
cctccaagcgaatgttaaaggcccttcaacttctttattgtcccggctctaatggtatcaactttgaacagcccatccataaagaacgaagaccaattca
attctggcagtca
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: