XB-CLONE-4353079
Clone Name: IMAGE:7026410
|
Gene:
cbr4
|
Species: Xenopus tropicalis
|
|
|
Sequence:
Full length sequence: BC080139 [+]
CCCCTGGCCCCGTAGTTGAATGGCGCTGCTGGAAAGATGACCAAGGTTTGTGCTGTTTTTGGAGGATCTAGGGGAATAGGTAAAGCTGTGTCTAAATTGT
TGGCTCAGAGAGACTATAAAGTTGCAGTTATATCTAGGGACCTGGAAGTAGCTAAAGCAGCAGCAGCAGAGGTTGGAGCTCACCTGGCCCTCAGCTGTGA
TGTTTCCAAGGAAAATGAGATCCAGGACACATTCAAAGAAATTACGAACAATTTGGGCAACGTTGACTATCTTGTTAATTCAGCAGGCATCCGTAGAGAT
GCTTTGTTACTAAGAACAAGATCTGAGGACATAAGGTCTCTCCTCTCTGTCAATCTGGTGGGCACAATCCAAACATGCAAGTTGGCACTAAGGAGTATGA
TTCAGCAGCAAGGAGGGGCCATTGTAAACATAGGAAGCATTGTTGGTCACAAAGGGAATATTGGTCAGAGCATTTACGGTGCCAGTAAGGAAGGATTAAT
TGGATTCTCCAAGTCCCTCGCTAAAGAAGTGGCAAAGAGGAACATCCGTGTTAATGTAGTCGCACCAGGATTCATTCACACTGATATGACTTTAGGTTTA
GAAGAAGATTCCCTGACTAAAATGGTCCCCTTGGGCAGGTTTGGAGATCCAGAAGAAGTTGCCCAGTCAGTCCTCTTTCTATTGGAATCCCCCTATATCA
CAGGGCATGTTTTAGTGGTTGATGGTGGCCTCCAGCTACAAATGTAGGATTTTTAATGTCCCAAAATATATATCAAAACATGTGAATTCCTGATGGTGTA
AATGGTTTAAACTTTTATATTGATTATTATGATTACTGTTTCTTTAAATAACTATTATAAGAATAGTAAATGTTGTTCAGATATTATTGTATATATAAAA
TCTCACTTAGGAAGTTGTTCAGTGTGGGTTTCATTTACCTTAAGCATACTTTATGTATGGCTGCACCAAGTTGCTTTTTACATTGAATAAAAAGAATATA
GTTATGGCTAATGAAGCAAAATCAGACAGAAGGACTCCACATTCTCAAGGGCTGTTTTTGTTAACTTACACATGGAAGGCTGCACCCATATACACTAGCA
AAATGTGTTTCCTGTAACATTATGCCATCCCCTGTGATCAGTTCTTAATTGATGCTAATTCACCATGAAGCAGGAAGATCGTCAGAAAGATACTTTTAAG
TTATGCAAAAAAGAACAGGAAGCCAACGATGGACTGGCCAGCATCTCAACAGCCCTCAACCCTAGTGAGCTTTTTGGGAAGAGCTTCACTGTAAGATCAG
CAGAAAAGTCCATGTCAGTCAGTCACATCCCTAGAAGAGCTACAGAAAGTGTGGGTTGAAGTTTCCCCTGCAAAAGGCCTCAAAAACAGAACACAGTCCA
AATTAACAGATATCAGTAAGGGCCCATGAAGTTTGGTTTGGGGATGATTTAATTCACTGAGCTGCATTCTTGCCCTATACCCTATTTGAGTAATATAATA
CATACAACCCATCTATTTAATCATATTTGTTATATCTAGGAATTATATGCACAGTCATGTGACACTGTATATCATTCTTTGTGGGTAATAAATGATTTTC
AGTGCAAAAAAAAAAAAAAAA
5' EST: CF783841 [+]taattaatccccccccccccccccctggccccgtagttgaatggcgctgctggaaagatgaccaaggtttgtgctgtttttggaggatctaggggaatag
gtaaagctgtgtctaaattgttggctcagagagactataaagttgcagttatatctagggacctggaagtagctaaagcagcagcagcagaggttggagc
tcacctggccctcagctgtgatgtttccaaggaaaatgagatccaggacacattcaaagaaattacgaacaatttgggcaacgttgactatcttgttaat
tcagcaggcatccgtagagatgctttgttactaagaacaagatctgaggacataaggtctctcctctctgtcaatctggtgggcacaatccaaacatgca
agttggcactaaggagtatgattcagcagcaaggaggggccattgtaaacataggaagcattgttggtcacaaagggaatattggtcagagcatttacgg
tgccagtaaggaaggattaattggattctccaagtccctcgctaaagaagtggcaaagaggaacatccgtgttaatgtagtcgcaccaggattcattcac
actgatatgactttaggtttagaagaagattccctgactaaaatggtccccttgggcaagtttggagatccaaaaaaagttgcccagtcagtcctctttc
tattgggatccccttataatcaaggggcatgttttaatggtttgatgggtggcctcccagccttcaaatgttaggaatttttaatggtctccacaatatt
atattcaaaaccatgtgggaattccctggaagggtggtaaatgggtttaaacctttttaaaaatgaatttattaaagaattaccggtgtttctctttgan
atatacctttttttttatagataataggtgaaatggtgtgggttcacgaaaaaataatatgtgggataatttataaaaan
3' EST: CF783840 [+]tttttttttttttttgcactgaaaatcatttattacccacaaagaatgatatacagtgtcacatgactgtgcatataattcctagatataacaaatatga
ttaaatagatgggttgtatgtattatattactcaaatagggtatagggcaagaatgcagctcagtgaattaaatcatccccaaaccaaacttcatgggcc
cttactgatatctgttaatttggactgtgttctgtttttgaggccttttgcaggggaaacttcaacccacactttctgtagctcttctagggatgtgact
gactgacatggacttttctgctgatcttacagtgaagctcttcccaaaaagctcactagggttgagggctgttgagatgctggccagtccatcgttggct
tcctgttcttttttgcataacttaaaagtatctttctgacgatcttcctgcttcatggtgaattagcatcaattaagaactgatcacaggggatggcata
atgttacaggaaacacattttgctagtgtatatgggtgcagccttccatgtgtaagttaacaaaaacagcccttgagaatgtggagtccttctgtctgat
tttgcttcattagccataactatattctttttattcaatgtaaaaagcaacttggtgcagccatacataaagtatgcttaaggtaaatgaaacccacact
gaacaacttcctaagtgagattttatatatacaataatatctgaacaacatttactattcttataatagttatttaaagaaacagtaatcataataatca
atataaaaagtttaacccatttacaccatc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: