XB-CLONE-4353087
Clone Name: IMAGE:7026029
|
Gene:
tnnc2
|
Species: Xenopus tropicalis
|
|
|
Sequence:
5' EST: CF783835 [+]
taattaatcccccccccccccccccccgaaaagctttgggggtctgtacagaagaggaaacagatacctcctgccatccttctcctctgcaggcatttgg
ggtgacctgccaagatgcctacagaccaacagcaagatgcgaggtccttcctcagcgaggaaatgattgctgagttcaaggctgcctttgacatgtttga
cactgatggtggcggtgatatcagcaccaaggagttgggaactgtcatgaggatgttaggacagaacccaaccaaggaggagttggatgccatcattgag
gaagtagatgaggatggcagcggcactatcgactttgaggagttcttggtcatgatggtgcgccagatgaaagaggatgcgcagggcaaaagtgaagagg
agttggctgagtgcttccgcatttttgacaagaatgctgatgggtacattgatggcgaggagttagctgaaatcctccgttcctcaggtgagagcataac
agatgaggagattgaggagcttatgaaagatggagacaaaaacaacgatggcaaaattgactttgatgagttcttgaagatgatggaaggtgtgcagtaa
gccgtaggacattcctctggaacgttgtgacgccaacctgtacattacactggcgtcttacttacatttcaggaagggacaagtcatcccggctcttttt
acgggaaccccccacaaatcctccccccctttcggaaagggtacatttacttctcctaaggggccacctttggcatctctggaaaaaaaaccctccttaa
atcccacctgggttttacctaattagaggcgttctc
3' EST: CF783836 [+]tttttttttttttttcaaaatacatcaccctttttatttacatgctgaattgtatgaatgtgaaaataacggatatgcaaaaatattagtgctttccact
gtgtctacgtgtcagcatgaggttggcagcttctgtgttattattaggcaaactagacagccctctagggtggaatggggtagccataagctagtgtgtc
tcaaaactttttaatgctgctatttatgtgagcattgagcaaaaaaagatactgctacatttgctttgcaccacaaatagatatctaaaggagtttctat
cctcttctgagtatgaatgcagcccagagatagcaaaggttcactaagagttttaatggtaactgtaatttttttttataaataacatatgagtcagaaa
aacaggaagatgaagagggtagagagcctgatagtaaacagtggattaagaggttctctgcaggatgcaaggtggccatagagagtaatgtacacatcaa
aagcgaggaggattgtgggaggtacagtaaaagagcaggatgacttgctccttcctgaaatgtaagtaagacgcagtgtaatgtacaggttggcgtcaca
acgctccagaggaatgtcctactgcttactgcacaccttccatcatcttcaagaactcatcaaagtcaattttgccatcgttgtttttgtctccatcttt
cataagctcctcaatctcctcatctgttatgctctcccctgaggaacgggaggatttcagctaaactcctcggccatccatggtacccatc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
Suppliers: