XB-CLONE-4353098
Clone Name: IMAGE:7026033
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Gene:
dazap1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783828 [+]
atgcagcctgagagaacccggccacgagaaggctggcagcaaaaaggacccagaactgaaaacagtaggtccaacaaaatttttgtgggaggaattccac
acaactgtggggaaactgaactgagggaatattttaaaagatttggagtggtaactgaagtggttatgatttatgatgctgaaaaacagaggccaagagg
ttttggatttataactttcgaggacgaacaatcagtggaccaggctgtcaacatgcattttcacgacatcatgggcaaaaaagttgaagtcaaacgggca
gaaccacgtgatagcaaaagccaaactccaggacctcctggatcaaaccagtggggaggtagagcaatgcaaagcacagctaatggatggacaggacagc
ctcctccaacgtggcagcagagctacagtccacaaggtatgtggatgccaacaggacagaccattggcgggtacggtccacctgcaggccgggggggtcc
tccaccaccaccttcctttgcaccattcctagtgtcaacaacccctggagcttttccaccaccacaggcctttcctcctggatatgccacaccaccttcc
attcggctatggctatgggccacccccctccccctcctgaacaattcgttctcttccgggagttccaccaccttccgggaacttccaaggggcagccacc
gtttatgcgttccccttcaaccttcccccaaggggaaagtcaaccttccaagaatcttggaagcaaaaacccccccccaatgtggtccaggccacggaaa
tttttccccttttttaacccccgaatttggggggtttaagggggacacaaaaaaacacattaaaatttgggggt
3' EST: CF783829 [+]tttttttttttttttagaagcaaggagttaaaatctgaactccagtgtcaccggaaaccctgccattaaattaaaaaataaacctgaatacatagtagaa
aattcatttttttgtttaaaaattcacaatctgtaaatgtatatattttcatctttaaacataaaagtttactatatggtaaaacaaaggctcagaaaat
ttccctcccaaaagagaaaaaaaaaaaaaacaaaataaaaagaacaaaaacaaaaaaaacccaaattaaacctgaagagttccattaaagctgcagatgt
tttcaaaccctgtttggaaccagtgactcattggctttatttgtgctgatgggtcagaaaaagatctgaaaactgtagtccaaatacccacagcttctcc
ttcaaacttagaacttccacaagtctatagcaggaatggtcttcaccacgtgattgtcacaagttcagaggtggaagtgtcagctcatcgtcactgtgca
caattctgcctgcccgatattagcgcctgtagggatggaatccttgcacattgtgattctgccctctgccaaaacacttcccccggcgggtgggcctccc
gatgctggtgtggagggtcctgacgtataaggggggttgctggttgaagtcagggaaaacttgcccatacccactcatgtcttgtccataaaccaactgg
ctaaaaaggaaaatcctgcttgacacctgggggggtttgctcaaatcctggaactgactgccctggggggaagttggaaggaaacgctaaacgggggctg
ccccctggaaatttccagggaagtgggtg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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