XB-CLONE-4353114
Clone Name: IMAGE:7026039
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Gene:
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783818
[+]
taattaatccccccccccacccccccagctcatttcctgtgccacacgggagtcaggcagtaggttgcacccatgcagcacctgttggacccaataatac
tcaacacaatgaataaagataagaagcaaatgactgagaagatattgaatcatgccctgggtattatattcttactcaccggcgaggtatgagcgatttc
tattccttgtgaagtattgcactaatttcttataaaatctttatattgtttcctgtatttagttataatatgagaaatgtgcttcactgtactccctgct
tcaagatgaaccatccttatattacttttaatttctatacaagttctcagcccaaaatgtatctttaatgccagatccagctcccctctttctcttgttt
ttagggcagtggcatagcacaggtaattgctcccttaggtctaggctattcattgtgggactgggaaacagggtcgtactgggccgttggggcaccaaga
aaaaaccctgtgggcaaagccgaccctgacctgatgtaggagtggaaggcaaataatttgaaaaattataaacattgaagaccaaccgccaaattgctag
gaaataggaaaacttctacaacataactaaaaaatttactggaataaggtggaaaccaccttccttttaaagctcaggtggggggggaaaggcaaaattt
gggcgatcattcaaaaccatttttcacgtggaaaaagaaaggcccccttggaaccaaggaacaagggggctaaaacccgggaagtccccccctccccccc
cgttatttctcaatatggggacaccaaattcttataaagcccccgggttttttcccttttggaataaaatgtccaagcgtgtgaaagggttccattttat
aaa
3' EST: CF783819
[+]tttttttttttttttcctccttggcaataagcacattttctggagctttgttctgtgtatttgctgggccagtgtctgaaccaacaggatcctgctgaac
agcattaccatgtttctccattttgtccacgtcattagttgccgccatgttttgatttctgtcacaaaggcctggaaattaaagagatttcaacaatatt
accaaataagggcttctatatataaaagcggccatacgcataccgtccgtgtaccctttttgcactggctgattggatgaggagtcacacatagtatggc
tgctttccttttattgtttgtgcaagctgctgtttcaatgcaccatgaactggccgacagggtccatttgctaaactgaataattccagaattgactgat
atccatatcaaagtcatacaaaacgttgtgattatattattggcatgtgcatgggcagcttaactcatagaaaggcttcaatagttcccataagccagtt
tcactctctcaagactctcaagtcaaacctctctgagctgtgatctgcctataatccccaagggcaaagtgtttaccgtacatttatatctgaagcagca
aatagagacatttggaccaccagtgtttttccccctaagtccatagacctcataagaaagtgttaccagagtgttcttgtgctggaacttctgaagactt
ctgattgtctgtttacaacatccgggaaaggctttcttggggtccttccaagaacctcccattccttccagggaaaaaataaactggcaacaattcccca
acacttttatttgggaaacttaaaaaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: