XB-CLONE-4353723
Clone Name: IMAGE:7026279
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Gene:
rpl19
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077657
[+]
GCCGAGCTGAAGCAGAGCGGCCGCCATGAGCATGCTCAGACTTCAGAAGAGGCTCGCCTCCAGCGTGCTGCGCTGTGGCAAGAAAAAAGTATGGCTGGAT
CCAAATGAAACCAATGAGATTGCCAATGCCAACTCTCGGCAGCAGATCAGGAAACTGGTTAAGGATGGCTTGATCATCCGCAAGCCTGTGACTGTCCACT
CCCGTGCCCGGTGCAGGAAGAACACCCTTGCCCGTAGGAAGGGAAGACATATGGGCATTGGTAAGAGAAAGGGTACTGCCAATGCACGTATGCCAGAGAA
GCTTGCTTGGATGAGGAGAATGAGGATCTTGCGCCGCTTGCTTCGCCGTTACCGTGAATCTAAGAAGATTGACAGGCACATGTACCACAGCCTGTATCTG
AAAGTAAAGGGAAACGTGTTCAAGAATAAGCGTATCCTGATGGAGCACATCCACAAACTAAAGGCTGACAAAGCTCGCAAGAAGCTTTTGGCTGATCAGG
CTGAGGCTCGCAGGTCTAAGACAAAGGAGGCAAGGAAGCGCCGCGAGGAGAGACTGCAAGCCAAAAAGGAAGAAATCATTAAAACACTTTCTAAGGAAGA
GGAGTCTGGAAAGAAATAAATCCCACGGCCCTGCCATTTGTGTATATAGAGTGCTGCTTTGTGCAAACTCAATAAAAACTCACCAACACCGCGAAAAAAA
AAAAAAAAA
5' EST: CF783432
[+]taattaatcccccccccccccgccgagctgaagcagagcggccgccatgagcatgctcagacttcagaagaggctcgcctccagcgtgctgcgctgtggc
aagaaaaaagtatggctggatccaaatgaaaccaatgagattgccaatgccaactctcggcagcagatcaggaaactggttaaggatggcttgatcatcc
gcaagcctgtgactgtccactcccgtgcccggtgcaggaagaacacccttgcccgtaggaagggaagacatatgggcattggtaagagaaagggtactgc
caatgcacgtatgccagagaagcttgcttggatgaggagaatgaggatcttgcgccgcttgcttcgccgttaccgtgaatctaagaagattgacaggcac
atgtaccacagcctgtatctgaaagtaaagggaaacgtgttcaagaataagcgtatcctgatggagcacatccacaaactaaaggctgacaaagctcgca
agaagcttttggctgatcaggctgaggctcgcaggtctaagacaaaggaggcaaggaagcgccgcgaggagagactgcaagccaaaaaggaagaaatcat
taaaacactttctaaggaagaggagtctggaaagaaataaatcccacggccctgccatttgtgtatatagagtgctgctttgtgcaaactcaataaaaac
tcaccaacaccngcgaaaaaaaaaaaaaaag
3' EST: CF783433
[+]tttttttttttttttcgcggtgttggtgagtttttattgagtttgcacaaagcagcactctatatacacaaatggcagggccgtgggatttatttctttc
cagactcctcttccttagaaagtgttttaatgatttcttcctttttggcttgcagtctctcctcgcggcgcttccttgcctcctttgtcttagacctgcg
agcctcagcctgatcagccaaaagcttcttgcgagctttgtcagcctttagtttgtggatgtgctccatcaggatacgcttattcttgaacacgtttccc
tttactttcagatacaggctgtggtacatgtgcctgtcaatcttcttagattcacggtaacggcgaagcaagcggcgcaagatcctcattctcctcatcc
aagcaagcttctctggcatacgtgcattggcagtaccctttctcttaccaatgcccatatgtcttcccttcctacgggcaagggtgttcttcctgcaccg
ggcacgggagtggacagtcacaggcttgcggatgatcaagccatccttaaccagtttcctgatctgctgccgagagttggcattggcaatctcattggtt
tcatttggatccagccatacttttttcttgccacagcgcagcacgctggaggcgagcctcttctgaagtctgagcatgctcatgggggccgctctgcttc
agctcggcggggggggggggggattaatttaactcggagtggcaaaaaaacaagtaacctttgtattcccgggccccggaaaaaaacccaaaggggcaat
ttgggacctggggaagggggaccccctgggtggaaagagaaaagggccaaaga
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: