XB-CLONE-4353788
Clone Name: IMAGE:7026306
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Gene:
abce1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783392 [+]
taattaatccccccccccccccggagccgcgtcgtgcgtttcacactggcacgcaacgctttcctacgccgggctagcgcgagaaggaaaggggcgccgg
ccattttggccggtttctcatcgtgtggcttccacgctgctggccggggagaagaggggaggtggtaggccgctttatctccctccttccccccagtgtt
taacatttccgccgtcacccatagctgaatatcttttcctgcggtgttaaagttggtgtccattgaaaaacaaagctacagttataccagacaatatggc
agacaaactaacgagaatagctattgtcaatcatgacaaatgcaagccaaagaaatgtcgtcaagaatgcaaaaagagttgccctgttgtaaggatggga
aaattgtgcattgaagtgactccacaaagtaaaattgtatggatttccgaaaccttgtgcattggatgtggtatctgtatcaagaaatgtccctttgtag
cattgtcaattgtcaacttgccgagcaatctggagaaggaaacaacccacagatattgtgccaatgcctttaaacttcacaggttgcctattccccgacc
tggagaagtacttggggttggttggtacaaatggtatcggaaaatctacagcattgaaaattttggctggaaagcagaagccaaaccttggaaagcatga
tgatccctccgactgggcagagatctttgacttattcaaggggtcagagttgcagaaattacttccccaaattttggagggatgacctgaaggccatcat
ttaacctccatatgtgggaccaaattccaaaaacctggccaagggaacctgtggggttaccaaattgggaaaaaaa
3' EST: CF783391 [+]tttttttttttttcttttaggtaatatttatttttcaaaaccttgaagtttaggctctctttaaaataagatcatgttctttatggcagcattgtatgta
aaccatcaaagaaatccagtttacaaaggtctaaaattggggacaattaatctgaatattgaaaagtgaataaattagattgccaggttctcaatccata
caataaattgtactttgcactttttatgaacttttcttatgtggccaatcacaacagttcttggtcagttttactttatcctgcaacataatgcagaagg
ttgtatgaaacaatgtttcatacatcagagaggcttgatttcttcggttgtagaaaggtgtgggttttagcttagtcatctaagaaaaagtagttgccac
tcttcttctgatccacatccttaatagaattcattttgtttattcttggtctgtagttgttaggatctctcctaaacgtgatttccagctgtgacaagaa
cttgttcatgccagccaatagtgtttgagggctgttagcaacagtatctttggaaggtataccatcaaacacaatgactctgtcagcaagataagtcgcc
attataaaatcgtgctcaacaataaaagccgtcctcttggcgtggagaaatgaatccttttgatttactcttggcggcctgtgaaaaacgctgtttcaga
aatcccagaaaaaggctggaatggcttccctccaattttagaataccaccatccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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