XB-CLONE-4353791
Clone Name: IMAGE:7026307
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Gene:
rfc4
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080504
[+]
GGCTTGCGTGTAGTTGTCCCGCGTTCTGTAACACAGTAGGCTGAGAGGCTGCAGCTACATTTTCGCGGGAGGTAGCTGTCACAATGCAGGCTTTTCTAAA
AGGAGGAACATCTATCAGCACCAAGCCTGTGAAAGATAAAGCATCTGCATCTGGCACAGGGAGCACCGGGGAAAGCAAAAAACAGAAGCCTGTTCCATGG
GTGGAAAAATACCGACCCAAATGTGTAGATGAGGTTGCCTTTCAAGAAGAAGTTGTTGCTGTACTAAAAAAATCACTGGAAGGTGCAGATCTCCCAAACC
TATTATTTTATGGCCCACCTGGCACTGGAAAAACATCAACAATTTTGGCAGCATCCAGGGAACTCTATGGGCCTGAATTGTTTCGCCAGCGAGTTTTGGA
GCTGAATGCATCTGATGAAAGGGGGATACAAGTGGTACGGGAAAAAGTTAAAAATTTTGCTCAGCTCACAGTTGGAGGGACACGTTCTGATGGTAAACCT
TGTCCCCCTTTTAAGATTGTAATTTTGGATGAAGCAGATTCCATGACATCAGCTGCACAGGCTGCCCTCAGGCGCACCATGGAAAAGGAGTCCAAAACAA
CTCGGTTTTGTCTCATATGCAATTACGTCAGCAGAATAATTGAACCTTTAACATCTCGATGTTCAAAATTTCGTTTCAAACCCCTGGCTGATAAAATACA
GACTCAGCGACTACTGAGTATTTGTGAAAAAGAAAATGTGAAAATTACAAATGAGGCAGTAAGTTGTTTGGTTGAAGTGTCTGAAGGAGACTTGCGAAAA
GCCATAACATTCCTTCAGAGTGCTACAAGGTTAACTGGAGGAAAGGAAATAACAGAGGAAATAGTGACAGAAATAGCTGGGGTAGTCCCTAAAGAAACAC
TTGATTGTGTATTAGTGGCCTGTCAGAGTGGTTCTTTTGAGAAGCTGGAAACTGTTGTCAAAAATCTGATAAACAATGGACATGCAGCCACACAGCTTGT
AAACCAGCTCCATGATGTTATATTAGAGAGAGGGGACCTTACAGACAAGCAGAAAGCATTTATTACAGAAAAACTTGCAGAAGTTGACAAATGTTTGGCA
GATGGAGCCGATGAATACCTGCAAATGCTGGGTCTTTTCGCAGTTGTAATGCAACAAATGACTTAAAATAGCTAATAATAATTTTTCTGGTTAATGACAA
CAGAGGTCTGAAATCTTTTGTAATGCTTCTAATAAATTTGTATAAAATATGTACCACTAAACAAAAAAAAAAAAAAA
5' EST: CF783390
[+]taattaatccccccccccccggcttgcgtgtagttgtcccgcgttctgtaacacagtaggctgagaggctgcagctacattttcgcgggaggtagctgtc
acaatgcaggcttttctaaaaggaggaacatctatcagcaccaagcctgtgaaagataaagcatctgcatctggcacagggagcaccggggaaagcaaaa
aacagaagcctgttccatgggtggaaaaataccgacccaaatgtgtagatgaggttgcctttcaagaagaagttgttgctgtactaaaaaaatcactgga
aggtgcagatctcccaaacctattattttatggcccacctggcactggaaaaacatcaacaattttggcagcatccagggaactctatgggcctgaattg
tttcgccagcgagttttggagctgaatgcatctgatgaaagggggatacaagtggtacgggaaaaagttaaaaattttgctcaactcacagttggaggga
cacgttctgacggtaaaccttgtcccccttttaagattgtaattttggatgaagcagaattcatgacatcagctgcataggctgccctcaggcgcaccag
ggaaaaggagccccaaaacaactcggttttgcctcactatgccaatttacttcggcagaaatatctgaacctgttaacctttcagatgtttcaaaattgt
cgtttccaaacccctgtgggtgactaacaatacaggacctcaccggatttacccgagccaattcccgtggaaaatggcacaattgcgtataaatttcacc
ccaccgacaggctatttaacaatttgccccctcgaacttgatagaca
3' EST: CF783389
[+]ttttttttttttttgtttagtggtacatattttatacaaatttattagaagcattacaaaagatttcagacctctgttgtcattaaccagaaaaattatt
attagctattttaagtcatttgttgcattacaactgcgaaaagacccagcatttgcaggtattcatcggctccatctgccaaacatttgtcaacttctgc
aagtttttctgtaataaatgctttctgcttgtctgtaaggtcccctctctctaatataacatcatggagctggtttacaagctgtgtggctgcatgtcca
ttgtttatcagatttttgacaacagtttccagcttctcaaaagaaccactctgacaggccactaatacacaatcaagtgtttctttagggactaccccag
ctatttctgtcactatttcctctgttatttcctttcctccagttaaccttgtagcactctgaaggaatgttatggcttttcgcaagtctccttcagacac
ttcaaccaaacaacttactgcctcatttgtaattttcacattttctttttcacaaatactcagtagtcgctgagtctgtattttatcagccaggggtttg
aaacgaaattttgaacatcgagatgttaaaggttcaattattctgctgacgtaattgcatatgagacaaaaccgagttgttttggactcctttttcatgg
tgcgcctgaaggcagcctgtgcagctgatgtcatggaatctgcttcatccaaaatacaatcttaaaaagggggacaaagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: