XB-CLONE-4353794
Clone Name: IMAGE:7026308
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Gene:
ap1ar
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783388
[+]
taattaatcccccccccccccgacagaaatgaaggccaaaggtgtgagcgcgttctgtggggactgagcaagggtgcgcaacttatatcctgtgggctgc
tgcattttgcgcgtttcggttcagtatgcgatggggatctgctggactcggtgctgcggcatcttccgaagagagactagccggctacagagagctggag
gcggatcaaaatatttcaaaactaccaccagaggcgagcactttacaattgagtttgaaaaccttgttgaaagtgatgagggagaaagtcctggcagtag
cataagacccttgagtgaggaagaagtatttgatctaaaggaaaagcattatgattctattactgaacggcagagaactgatgatatcaggctacaaaat
gagttagccttacaagaggagaagttaaaactagaagaggaggctttatatgctgcacagcgggaagcagcgagggcagccaagcaaagaaagattttgg
agcaacacaggttgcagtggataaagcaaagagcactagcagctaagaatggagaagatgcaagctctgtttcagaagattttgaaacctacttgcagaa
tctgaagtcacaatatggagcttttaagaacagcagattgtcttctgaagcaaatgtattgacacccaacacagaaagcaactgtgatttaactacaaaa
aaaagtcaacaagcggggaatgatgataacacatctctttgacttgagtgggaaaatgaagaagggattgaacaggatgattcccaatgaaagaacggct
caaaaaacaaaagaggaaattctttaaaaacagcccttttaaaaataattggcaaaaaaaccctgggaaaattcatgggaaaccctcctggccttctgga
tggaacctaaaaaagggggccttccaaagtggggaaaaaaatagaacttttttgtaaaaaattgcccaaa
3' EST: CF783387
[+]tttttttttttttttagttttcacttagtgcagataatttattagtgatcctagacaaaaagcagcaaatactaactagatggacccgtcacaataaaag
gaaattaaaaacagtgaaaattgcacttgttctttttaaaactttccacctttctaccaatctcccttttatcatttaaaaaaaaaaaaaaaaaaaatga
aggctccaagaaaaagtgtacattcattgtaatccataaggaaggagaatcttcatctgtttggtcattaatatatttatgagcgagtgtctttacctat
tctgatgctgagcagatagttctgtgactggatttacaaaccctgcatactcagagttcccattgtcatccatttctgcacttacaaagtcattttccca
ctcgagcccatttgagtcatcagaagcagaggctccatgacttccaggcttcttgccactatgttttaaggctgctcttaagatgtcctcttctgtcttt
gagcgctctttcattgggatcatcctgttcattccttcttcatcttcccactcaaggtcaagagatgtgttatcatcattcccgcttgttgactttgttt
ttgtagttaaatcacagctgctttctgtgttgggtgtcaatacatttgcttcaaaagacaatctgctgcttctaaaagctccatatttgtgacttccgat
tctgcaagtaagttttcaaaatctttctgaacaaaagctggcattcttctccatttcttagctggccaggggctcctttgctttaatcccccggcaaccc
tgtggtt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: