XB-CLONE-4353800
Clone Name: IMAGE:7026315
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Gene:
atp6v1b2
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080455
[+]
CGGCAGTCTGAATCGCTGAGAAGATGGCGGTGCGTGTAATGCGGGGAGGTGAGGGCGGCCCGGGGAATCCCATAGGTGCAGCGGCCAGAGAACATGTACA
AGCGGTGAGCCGAGACTACCTGTCCCAGCCCAGGCTGACTTATCGCACTGTGTCTGGTGTTAATGGGCCCCTGGTTATCCTGGACAATGTCAAGTTCCCC
AGGTACTCAGAGATTGTTCACCTGACTCTCCCCGATGGCACAAAGAGAAGTGGGCAGGTACTCGAGGTCAGCGGATCCAAAGCTGTGGTACAGGTGTTTG
AGGGCACATCCGGCATTGATGCAAAGAAAACCTCATGTGAGTTCTCTGGGGATATTCTCAGGACACCAGTATCCGAGGACATGTTGGGACGTGTATTTAA
TGGTTCCGGAAAGCCTATTGATCGTGGACCCAGTGTTTTAGCTGAGGATTATCTAGACATAATGGGGCAGCCCATAAACCCCCAGTGCAGAATCTACCCT
GAAGAAATGATCCAGACAGGGATCTCTGCTATAGATGGTATGAACAGCATTGCCCGTGGTCAGAAAATCCCTATTTTCTCTGCTGCTGGTCTTCCACATA
ATGAGATTGCAGCTCAGATCTGTCGTCAAGCAGGTCTGGTGAAGAAATCCAAAGATGTAATGGATTATAGTGAGGATAACTTTGCCATAGTCTTTGCTGC
TATGGGGGTTAACATGGAGACTGCCCGATTCTTCAAGTCTGATTTTGAGGAGAATGGCTCCATGGACAATGTGTGTCTCTTTTTGAACCTGGCCAATGAC
CCCACAATTGAGCGTATTATCACCCCACGTCTTGCCCTTACCTCTGCGGAGTTCTTGGCCTATCAGTGTGAGAAGCACGTACTGGTGATCCTGACTGACA
TGAGCTCCTATGCTGAGGCTCTGAGAGAGGTATCTGCAGCTAGAGAAGAAGTCCCTGGTCGCCGAGGCTTCCCAGGTTACATGTACACTGATCTGGCTAC
AATCTATGAACGAGCTGGGCGTGTGGAAGGAAGAAATGGCTCTATCACTCAGATCCCTATCCTTACCATGCCCAACGACGATATCACTCACCCCATTCCT
GACTTGACTGGATATATCACTGAGGGACAGATTTACGTGGACAGACAGTTACACAACAGGCAGATCTACCCACCCATCAATGTGCTTCCCTCACTTTCCC
GTTTGATGAAGTCGGCCATTGGGGAAGGCATGACAAGAAAGGACCATTCAGATGTATCTAATCAGCTGTATGCCTGCTATGCCATTGGAAAGGACGTACA
AGCCATGAAGGCCGTAGTTGGAGAGGAGGCTCTGACCTCCGATGATCTTCTGTACTTGGAGTTCCTACACAAATTTGAAAAGAATTTTATTGCGCAAGGT
CCTTATGACAACCGCACAGTTTACGAAACGTTGGATATTGGATGGCAGCTTCTTCGAATCTTCCCCAAAGAGTTGCTGAAGAGAATTCCCCAGAGCACCC
TGGCAGAATTCTACCCCCGAGACTCTTCTGCCAAGCACTGAGCAGCCGCCTCATCTATCTCCATGTGTTCTTTACCCTGCACTTACTCCCCTCTCCTCCA
TGTGTGCCGTTTACAGGTTCTTGTCCATCATGGAGTCACTCACAGGAAGGGAGCAGCGACGAGATATTAGCCACAAAGATAGAATGGCAATACAAAAATC
CCACTTTTATTCTATCCATTGACTGTGGATTTCATTCATGTCAAATCTTAAGCATCTCAATATGATCCAGTAGCCGCGGTTTTGTACGCTAATGTGGTGC
AAGATGAAGTGCTCTATGTTCCCCTTGTGTGGCATGCGTATATTTATTGTGTAGCAGTGCCTGTGATTTTACCGGGGGCAGTTGATACTAAATTCTAGAA
ACCCACAAGCGACATTATCGTGGCAAATCCCTAATTACAAACTAGGCAAAAAGCCCTGGAAGCACATGTGTTTATAGGAATTTAGCCCTGCGCATGTACC
GGTTGGTTATAGTGATGCCCAGGGCTTTTGCCATTTCCAGCACTGTTTCCTGCTGCCTGACTCCATGCCTGTCCCTTTGCCCACCCATAGGCACCAGAAA
CTGGTAGAAGAGTGCAGGGAGGCAATTGTCTTTGGTACCAGATTCCTCCTGCGTTCCTTTGGATATTCTCCCCCCCCCTCCCTTTCTGTTTATTTGCTTT
TTCCTCTGTTATCTAACTCCCTGCAGGACCTACATCTCCCACACAGTCTGCCATTTTTACTTGCTCCGAAATGTTAGATTTTCCGTCATCCTTCCTATTT
CAAGATTGGTCTTATTTCGGCACGCCCTTCCCAGTCTCTCAGAATGCAGGCTGTTACTATGGGGGGAAGTTTTGTGTTATTTTATGTGAATTTGGTGCAA
TAGTTTGCTGTCTGGTTCATTCCATCTGTATACGGTGTATAAATTTATTGCTGAATGTCAAATCAAAGTAAATATTACATTTAATGTTAAAAAAAAAAAA
AAAA
5' EST: CF783383
[+]taattaatccccccccccccccggcagtctgaatcgctgagaagatggcggtgcgtgtaatgcggggaggtgagggcggcccggggaatcccataggtgc
agcggccagagaacatgtacaagcggtgagccgagactacctgtcccagcccaggctgacttatcgcactgtgtctggtgttaatgggcccctggttatc
ctggacaatgtcaagttccccaggtactcagagattgttcacctgactctccccgatggcacaaagagaagtgggcaggtactcgaggtcagcggatcca
aagctgtggtacaggtgtttgagggcacatccggcattgatgcaaagaaaacctcatgtgagttctctggggatattctcaggacaccagtatccgagga
catgttgggacgtgtatttaatggttccggaaagcctattgatcgtggacccagtgttttagctgaggattatctagacataatggggcagcccataaac
ccccagtgcagaatctaccctgaagaaatgatccagacagggatctctgctatagatggtatgaacagcattgcccgtggtcagaaaatccctattttct
ctgctgctggtctttcacataatgagattgcagctcagatctgtcgtcaagcaagtctggtggagaaattccaagatgtaatggattatagtgagggata
atttgccataatcttttgcgctagggggggttaacatggaaaaccgccccgattccttcaagtcctgaatttttgagggaggaatggcctcccctgggaa
aaaagggggggggccccccttta
3' EST: CF783384
[+]tttttttttttttttaacattaaatgtaatatttactttgatttgacattcagcaataaatttatacaccgtatacagatggaatgaaccagacagcaaa
ctattgcaccaaattcacataaaataacacaaaacttccccccatagtaacagcctgcattctgagagactgggaagggcgtgccgaaataagaccaatc
ttgaaataggaaggatgacggaaaatctaacatttcggagcaagtaaaaatggcagactgtgtgggagatgtaggtcctgcagggagttagataacagag
gaaaaagcaaataaacagaaagggagggggggggagaatatccaaaggaacgcaggaggaatctggtaccaaagacaattgcctccctgcactcttctac
cagtttctggtgcctatgggtgggcaaagggacaggcatggagtcaggcagcaggaaacagtgctggaaatggcaaaagccctgggcatcactataacca
accggtacatgcgcagggctaaattcctataaacacatgtgcttccagggctttttgcctagtttgtaattagggatttgccacgataatgtcgcttgtg
ggtttctagaatttagtatcaactgcccccggtaaaatcacaggcactgctacacaataaatatacgcatgccacacaagggggaacatagagcacttca
tcttgcacccacattagcgtacaaaacccgcggctactgggatcatatttgagatggcttaaagattttgacaatgaaatgaaaatcccacaagtcaaat
gggataagaaataaaaagt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: