XB-CLONE-4353808
Clone Name: IMAGE:7026318
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Gene:
actg1
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783379 [+]
taattaatcccccccccccccgctcattcagccggtggcagcggacttgacagctacttcccgctctttgtaacaacttgccagctttgcatttctcctc
ttaaggaaacctccagcaccaagaaataaaatggatgaggaaattgcagcactcgtcgttgacaatggctctggaatgtgcaaagccggctttgctggag
atgatgctccccgtgctgtgttcccctctattgttggacgcccaaaacaccagggtgtaatggttggtatgggccataaggacagctatgtatgtgatga
accccaatgtaacaggggtatccttacccttacgtatccaaatgaacacggcaattgttacatactgggatgccactgagaatactcggcctcacatttc
ccatctacgatcttcgtcgtaccccctgaatacccaccaaatcccgcttacagaaagcgccctcgcaaccctaaaagccctacgaaaataaaaccatgac
ctctgaatctttgttacggaagaattttttacccccctcattttcaagagaaagtccatccaaataaaaacgcctggtgtcgaggccttataagaaatag
cccccctaagggtccctataatttttggcctacacccgtggtttgtatacctctctattttatagggaggcttccctttaccaacaaagaacaactatat
ttcatttaaatggtgtttatccgcatttgtacccccttgatgtgtgttttctttccgtctctaggaaccccgggccattgtgtcacttttaacgcttgaa
caataactgtaatactccacatatagaataccccatccccccatataataacccgctcatttatttattacaataaccccgccagtcttacaaattttac
cgcactttcttttttt
3' EST: CF783378 [+]tttttttttttttttctttttttatttactgcagcgctttattttatccttacagaacggtgttttggaagttacaattctcatctggatgacatacaaa
ttttggttttctacgttgtgagaacattaaggcaacatacaaaaaaatttacataaattaacttggatgcataaatttacaggtgttaactgcaacattt
gtaactttcttcagacccattttgaatgttgggaatacaacctgggttaagaaaaagactggttcttgggcctgagtgtcaatttccaaccttactataa
actggaaagatagaagagattccttcatgaactgaccagcctccgacatgaaacaatggagtattaagcgcttaactgtgtgtggaagtctaaacctcag
ataccctgtacacatctccaaaactcgacttctccataaacagcccctgtaacctagaggagaagtgtcattcgcaatactgtcgcagatcggcagcctt
tcacagaaatgcttctttctacgtaaagcttagcttaagtgtcacccagactgtatgccaaccaggcaatataagagatgtgtacagggtacaatatgct
agcattgatgccacangaatgatttgaagagacaagcacaagtcttgcattacaaaatcccccaacagtgactatgtgtggcttcaattcctaggataag
ttcatttggaagttggccctttttccagttttcaagaggctagcatggagcgtacacatttgccaaggcaaaattaatactttggaaaaactcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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