XB-CLONE-4354039
Clone Name: IMAGE:7026504
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Gene:
krt70
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783238 [+]
taattaatcccccccccccccgatcagctcatacaggggaacacatcactgctacattgggccccttaacattacagcatcacaatgagtttctctaccc
gaagtgtctctcaatcctcccgctatggtgttctggcatcacccatggtgaatcgggccctaagtgtggcaaggggtgcccagactgtccggatgtcctc
tgctaaactgaccagctctgcgtttgccggaccctctgcccttgatgttagctcctttggcaacaatggcaaggagacaatgcagaacctgaatgatcgt
ctggccagctacctggaacgggtccgttccttggagcaagccaaccatgaactggagctgaatatcagggagtactgtgagaaaaaaagctgccattggc
tcccctgatgttaacggttactataacatcatcaactaactgcgctcccagataaatgatgcaagcatttgacaagcccgggctggtcctccatatcgac
aatgccaaactggtaagctgatgtacttcaaaattcaattatgatttcaaacctggccatttaggaattggaattcaaaaaaccgacaattaaaggaagt
ggggcaaaattccttcaattaaacatgaccccaccaaataaaacagtaaaatgtaagattgggataattttaaaaagtctcgaaaggaaaacaagctgga
atttacacctgtataaaacacaaatctatggaataaagggaaacttttggctgtttttggctaaggggtccagggggacttatatagtccctaagtgttt
gcagagaaccggatttgggaatgtgcttgagctgcctactcaatatagaaatctgtgccaccagaaattaaaggttacttgaatatgatatagggtttac
gatataattgaaataaatttaatatgaaaaaacatgaacactccgcaaagaagaagtgcgaaacgtctctgttttttcccttaacgtgcccantattttg
catttttccttctcccttttaactatgttatttatacatactttgtatcaacgatggaactatttgg
3' EST: CF783237 [+]tttttttttttttttgttttaacttagaattctttatttggatgcattgtccctgaggaagagttaaacacaaacagcctaggtttgcattacatgacat
ttattttgctaatttctcttctgctttcatatcttctctgtcatattaaacatttacgtataggctacattttaaaatgaagcttcttggttgccttcaa
gactatgttatttcaactcttctgctgccctttctcaggtacaggctcttaattcttaagattctttttaggatttctcagtcacttcctcgaccctaga
agagaccactttcccgtcgactacttcttcgatcaccgtcttcacctttttgatggagctcttgctttgttccttactgacgatggtaacagctttctgt
gtttcagtctgggaaaatctggtgtcttctccttctagcaggcgtctgtaggtggaaatttccatttccagccttgtcttggcatcgagcaacaacttat
actccaaagactggcgctcggcatctgaccgtaagttttgaaggtctaattctatttgcgtgatcatggcctgaaggtggttgagctcagctccatatcg
ggcctcggtttctgccaaagtgccttccagtgctcctttcatgctcaagagtgactgaagttcaatctccaggctctgtattgtgcgcttcacgtctgta
atctgcgacttaaatgtctggagagcagctgtgttggttggcaacttctttgtttgaaatttcgctcttgtctctgtaccaggctttcacc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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