XB-CLONE-4354159
Clone Name: IMAGE:7026558
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Gene:
arrdc3
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783164 [+]
taattaatccccccccccccccgaggcgtctttgtaaagagaccatagaagagagcactgtgacagcagctgctgctgttacttctcctacctgttcttc
ccgatgagctggccgctgggcgagcactgagcctgcctgcagtctctgcacggcaaatgcatactccagtgctctggcaacatcgccattgatgtgaaat
cggaaacttttgtctcccccccagaatatttttatagcccccttttacaatcatggtgctggggaaggtgaagagtttgaccataagctttgactgcctc
aatggcagccacgtccccgtctattccagcggggatgcagtctcaggaagggtcaatctggaagtgacgggggaaattcgggtgaaatccctgaggatcc
atgccagggggcatgcaaaagtcagatggacggaatcaaggaacgctggctccaacactgcctatacccagaattatactgaagaagtggagtatttcag
tcataaagacatcctgattgggcatgagagagatgacgataattcagaagaaggactaaccactattcattcaggaaggcatgaatatgcatttagcttt
gagcttccacaaataccacttgctacctcatttgaaggcagacatggcagtgtgcgctactgggtgaaagcggaattgcacaaaaccctggcttctacca
gttaaatttaaagaagggatttcccggtcttttgaacacctagaatatcaacccctcccttcatttactgggcttcccccaagcggggccacaaaaagaa
aaagaaccccgggggttggcttgggttaatggaccctccaggggtcccaatttattcctttaaattggcaccaaaataa
3' EST: CF783163 [+]ctttgttactactaggatgtgcatcctcatctcttgaccacatacgaatgcagcagcttctcgactcggcccacaagacggtgggagagcaaccgtatgt
atacagcacacacaccttataatgcagtatatgctgtaaccatgaccagaggctgattaagtctgccacttccaaatcgaaaaagcagtttcctttgaaa
gtgcgcctccaacataactgccataacttaacaggcagttaagagtcttgtctgtaaggaaaaagaatccctcagcgagatgggcaggaaggatgctctt
ctgtaggctgatctggattaggatcaacctctgaatatagtggggggggaagaaaacgaaattcttggatgtatgcaaatagtggtccttgaagagccaa
ttcaaaattgtcacaagcatttgaagacatcagattctgcctttgctcttctgtgacaacttctgcatagctgggaggcgcttcaagtctttctggcaat
gtcaaaccaagccaattatttatgctgtactggctgctcacactggatgtccggcttccaaatggatgcagtgggaaagtgcccattaccaatgggaaat
taaggaataaattccttagccccctggaatatccacatttacccttaagagaaaattccacacggattaatgctacagtccaatgatagaaaggggaaaa
tgggggggaattttcaagcaacttgccatttcaagttcttttgttttcccctgaaaaacaagagactctcccctctcaaagtgtgggaaaaaatttgctt
ttacttcccctacaattt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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