XB-CLONE-4354287
Clone Name: IMAGE:7026616
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Gene:
ccz1b
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082526
[+]
GGACAGGAAGCGAGGAGCCTGTAGTTGACAGCTTTGCGGGTCGGGATGGCATATGCAGTCCAGGATAAACGGTTTGCTCCATCCTTACTAAGCTTCTTCA
TATATAACCCAAAGTTTGGCCCACGAGAAGGAGAAGAAGAAAAAAAGATTCTCTTTTACCACCCAAATGAAGTAGAAAAAAATGAATAAATTAGAAATGT
CGGCTTATGTGAAGCAATCGTGCAGTTTACCAGGACCTTCAATCCAACAAAACCTGCTAAATCTTTGCATACTCAGAAAAACAGACAGTTTTTTCATGAG
CCAGAAGAGGGCTTTTGGATGGTCATGGTAGTGAAAAATCCACTTGCAGAAAAGCAAAAAGAAGGCAAGACAGTGTTGGAATACCAAGAAGATGAGTTAT
TAGATAAAGTTTATTCCTCAGTGCTTCAACGGTGTTACAGAATGTACAAGCTATTTAATGGCACTTTCAGCAGGGCAATGGAAGTTGGGCAGGTTGAACT
GCTTAAGGACAGACTGGAGAAGTTCTTTCATCGGTATTTGCAGACATTACATTTGCATTCCTGTGACCTTCTCGATGTTTTTGGCGGCATCAGCTTCTTT
CCACTGGACAAGATGACATACCTAAAAATCCAGTCTTTTATTAACAGAATAGAAGAGAGTCTGAACATAGTGAAATACACAGCTTTTTTATACAACGACC
AGCTTATATGGAGCGGGTTAGAACAAGAAGATATGAGAATCCTTTATAAATACCTCACTACTACCCTTTTCCCTAGGTATACGGAACCAGAGCTAGCAGG
TAGAGATTCACCCATACGCCCTGAAATGCCTGGCAATTTACAGCATTATGGAAGGTTTCTCACTGGACCCTTGAATCTCAGTGATCCCGAAGTCAAATTT
AGGTTTCCAAAAATATTTGTGAATACAGAGGACTCTTATGAGGAACTTTATCTGATTGTTTACAAGGCCATGAGTGCCTCTGTGTGCTTCATGATTGACG
CGTCTGTGCAGCTAACAGTGGATTTTTGTCGAAAATTGGACAGCCTTGTTGGACCTCAGCTTACAGTGCTGGCGTCGGATATATGTGAGCAATTTAATAT
CAACCGACGGATATCAGGGTCTGAAAAGGAACCCCAATTTAAATTTATTTATTTCAACCATATGAATCTAGCAGAGAAAAGTACAATCCACCTGCGGAAA
ACGCCAAGTATGTCCCTGACCTCAGTGCAGCCTGAACTAATGAAGATTCTAGGAGACATCAACGGTGATTTTACAAGAGTGGATGAAGACGAGGAGATCA
TTGTAAAAGCTATGAGCGATTACTGGGTTGTTGGCAAAAAGTCTGATCAGCGAGAATTGTACGTTATATTAAACCAGAAGAACTCCAATCTAATTGAAGT
AAATGAAGAAATCAAGAAACTCTGTGCAACCCAGTTCAGTAATATATTTTTCCTGGATTAACCTGCATGTGTTCAGCAGCAGGGTGGCATGCAGCATTTT
CAAAAATGGCCCGCATCAGCCTTTTTGTGTTCCATGGACAGAATATTGGTCATATTATTGACTGGAATGACTGTTGTAAGGCAATACTTGCCTTGTAAGA
CTCGCATTTCTATCCTTAGGTTCAACGAGCAGACTGTTTTAGATTTTAATTATTTATGTGAAATGTCGCAGAAGTCTCCACGCTAAAACGGCTGTACCAT
TCCATTTCTGTTACTTTAATACATGACTGGAAAAAATAAATCCACTAACTGTTGTTATACTTTTAAAAAAAAAGAAAAAAAAAAAAAAA
5' EST: CF783084
[+]taattaatcccccccccccccggacaggaagcgaggagcctgtagttgacagctttgcgggtcgggatggcatatgcagtccaggataaacggtttgctc
catccttactaagcttcttcatatataacccaaagtttggcccacgagaaggagaagaagaaaaaaagattctcttttaccacccaaatgaagtagaaaa
aaatgaataaattagaaatgtcggcttatgtgaagcaatcgtgcagtttaccaggaccttcaatccaacaaaacctgctaaatctttgcatactcagaaa
aacagacagttttttcatgagccagaagagggcttttggatggtcatggtagtgaaaaatccacttgcagaaaagcaaaaagaaggcaagacagtgttgg
aataccaagaagatgagttattagataaagtttattcctcagtgcttcaacggtgttacagaatgtacaagctatttaatggcactttcagcagggcaat
ggaagttgggcaggttgaactgcttaaggacagactggagaagttctttcatcggtatttgcagacattacatttgcattcctgtgaccttctcgatgtt
tttggcggcatcagcttctttccactggacaagatgacatacctaaaaatccagtcttttattaacagaatagaagagagtctgaacatagtgaaataca
cagcttttttatacaacgaccagcttatatggagcgggttaaaacaagaagatatgaaaatcccttataaatacttcactactaccccttttcccctagg
tataacggaaccagaagctagccaggtagagattcccccccttacccccctgaaatggctgggcaa
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: