XB-CLONE-4354368
Clone Name: IMAGE:7026653
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Gene:
ywhab
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084514
[+]
CCCCCGATCTGCAGGAGAGGAGGAGAACGGTGAGGGAAGCTGAGCTAGCCCGGGTCGAATTACTGAATCTATTGGCAGGTCGCGGATTTTTGAGATCTCA
CCATGGACAAGAGCGAACTGGTACAGAAGGCTAAACTGTCTGAGCAGGCCGAGCGTTATGACGACATGGCAGCTTCCATGAAGGCTGTGACCGAGCTGGG
TGCCGAGTTGTCCAACGAGGAAAGAAACCTGCTATCTGTCGCCTACAAAAACGTTGTTGGCGCCCGACGCTCCTCCTGGCGTGTGATCTCTAGCATTGAG
CAGAAGACAGAAGGAAATGATAAGCGGCAGCAGATGGCACGAGAGTACCGTGAGAAAGTGGAGACAGAGTTGCAAGATATCTGCAAGGATGTTCTGGGTC
TTCTGGATAAGTACCTTGTACCGAATGCAACCCCACCAGAAAGCAAGGTCTTCTACTTAAAAATGAAAGGGGATTATTACCGATACCTTTCAGAAGTAGC
TTCTGGAGACAGTAAGCAAGAAACTGTAACCTGCTCTCAACAAGCCTACCAGGAAGCATTTGAAATTAGTAAAAGTGAGATGCAACCAACACATCCTATT
CGACTCGGACTGGCTCTAAACTTCTCTGTCTTCTACTATGAAATTCTGAACTCTCCTGAGAAAGCATGCAGTCTAGCAAAATCGGCATTTGATGAGGCAA
TAGCTGAACTGGACACGCTGAATGAGGAGTCTTACAAAGACAGCACTCTTATCATGCAGCTACTAAGGGACAATCTTACACTATGGACCTCAGAAAACCA
AGGCGAAGAGGCTGATAATGCAGAGGCGGACAACTAACAACGGCCACCCATATTTTTTCCTTTTTTTCTGTTCAGTCACTCTGTTTTGTATACACATATA
TCCCTTTTTGTGCAACAAGTTAAAAGAATAGTACATCGACTTTCACAACCTCAGTAGCAGAACAATATGTGGATTAGGAAACAAGACTGGCGTCTTTACT
CAATGTAGGAGTAATACATTCTCAGTCTAAAAATATGGCATTCTATTTCTTTTTATATAAATATAAATGATTTAAATATGTAATTGTGTCCCTTTTTTTT
TTTCTCCCCTCCCTCCCCCCCCCCTTTTTTTTTTAACTGAACACTGTGATTTACGGTTACGGAGTTTTGTTATAATTTCCAATTGGCAATCACTATAACC
ACATTAGAACCTTTCATTGCCTCGGCAGTAACAACAAAGTCGACGTACAATTCTGTTTTAACTGTTTGACAGAGCGTTTGTGACCATACTGTGACCCTCG
GGATGGAATGGGTTCGTACCATAGTGTATTGCAGTTGTTTCAGTGATAGATATGTTTTGATCGACATGGTACATATTGAACCTAGTTTGTAAGCCTGAAT
ATAATCAAGTCGTTTTGGGTTTTTTTATTTTATATATATATAAATATATTATATATATATATTTAGTCACAATTTTTGAAAATCGATGTAATGCTCACTT
AGCCACTGTAACTCAGAAAAAAAAAGGTGGAAAAAAAAAAAAAAAA
5' EST: CF783035
[+]taattaatccccccccccccccccccgatctgcaggagaggaggagaacggtgagggaagctgagctagcccgggtcgaattactgaatctattggcagg
tcgcggatttttgagatctcaccatggacaagagcgaactggtacagaaggctaaactgtctgagcaggccgagcgttatgacgacatggcagcttccat
gaaggctgtgaccgagctgggtgccgagttgtccaacgaggaaagaaacctgctatctgtcgcctacaaaaacgttgttggcgcccgacgctcctcctgg
cgtgtgatctctagcattgagcagaagacagaaggaaatgataatcggcagcagatggcacgagagtaccgtgagaaagtggagacagagttgcaagata
tctgcaaggatgttctgggtcttctggataaataccttgtaccgaatgcaaccccacccagaaagcaaggtctttctacttaaaaaatgaaaggggatta
tttaccgataccttttcagaagtaactttctgggagaacgggtaaggcaagaaaattgttgaccttggctctccaacaaaggccttacccagggaaagcc
ccttttggaaatttgagaataagagttggaggattgtgaacccccaactacagttccctatactaccaaacttcggggaatttggccctccttaataact
ttcctcttggtctttctctacatattgaaaaagttctttgaaaatctctccctgggaaaaaaaagacgtggccaatctcccaagcaaaggattcgggccc
attttggattgcaaggggaaattagtcatggaaattgggggaccacacgtgtgatttgggagaaagttcctttctccaaaggaataagcgcccctcttct
catacaatggctagaccatgccttaaggggggagaaatttatttgaccctccataggcaccctcttacaaaaagacgtggagggcgaaatataaggcgtg
tattaatatttccctaaagggcgtggacactcatcttgaaagaatgggtcggatcgccctatatttggtt
3' EST: CF783034
[+]tttttttttttttttccacctttttttttctgagttacagtggctaagtgagcattacatcgattttcaaaaattgtgactaaatatatatatataatat
atttatatatatataaaataaaaaaacccaaaacgacttgattatattcaggcttacaaactaggttcaatatgtaccatgtcgatcaaaacatatctat
cactgaaacaactgcaatacactatggtacgaacccattccatcccgagggtcacagtatggtcacaaacgctctgtcaaacagttaaaacagaattgta
cgtcgactttgttgttactgccgaggcaatgaaaggttctaatgtggttatagtgattgccaattggaaattataacaaaactccgtaaccgtaaatcac
agtgttcagttaaaaaaaaaaggggggggggagggaggggagaaaaaaaaaaagggacacaattacatatttaaatcatttatatttatataaaaagaaa
tagaatgccatatttttagactgagaatgtattactcctacattgagtaaagacgccagtcttgtttcctaatccacatattgttctgctactgaggttg
tgaaagtcgatgtactattcttttaacttgttgcacaaaaagggatatatgtgtatacaaaacagagtgactgaacagaaaaaaaggaaaaaatatgggt
ggccgnttgttagttgtccgccctctgcattatcagccttcttcgcccttggtttttctgaaggtccaatagtggtaaagatttgtccc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: