XB-CLONE-4354377
Clone Name: IMAGE:7026656
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Gene:
psmb2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF783029
[+]
taattaatccccccccccccccccggagcgcgcccattcccggccttgtgctgatttgctgtgtaacgtcccgaggccgtaattagagactcataactcg
ggtaaccaacagcggcgggaatatggagtatctgattggcattcagggcaatgacttcgtacttgtggctgcggacacagtgtgtgccaacagcatcatt
aagatgaagcacgatgtggacaagatgtttaagatgagcgagaagatcctgctgctgtgtgtaggagaggcaggtgacacagtccagtttgcagagtaca
ttcaaaagaacgtgcagttgtacaaaatgaggaacggctatgagttgtctcctactgcagccgcaaacttcacacggagaaatctggctgactatcttcg
cagcaggacccccttaccatgtcaatctgctgcttgctgggtatgatgaacatgacggaccctcgttgtattacatggatttacctagccgctctaggca
aaaacgcgttttgctgcacatggctaccgggctttacctaacccttcagtattctgggattcctacttccaacccggacttgaaaaggaggggacgcaga
aaaacttctaaaagaaaatgtatatgcataatcttcccgagagccgcttttatacctgaaaccctggccttccttttaaaagttgggggggtccctccta
ttaaggagtgggggacccaatggactttgggaaacaaatttcctgcctttaggggcccttttaaccctttcccctcttcccttggaatcagccccccccc
ccctttttccgttttttaaagaaaaaaaaggggtaacatttttccaccactcgtgatttctaataacacaataaaatttagcccccgtgggggagtattt
ccgcttggtaggggacctacccccaaggatgaaaatataaaaataataataaggagacccccccacgaggttctccaaaaggggggactgccccctcctg
tcggtgtgagagaaaatattagn
3' EST: CF783028
[+]tttttttttttttttcctgggagtcccacatgcaaatgcacagggtaatttatttgtttcatacagtggtacaaattaaccttaatatataaaaagaaaa
aggaggaggcgagtcagggagaggtaaggttaaaggcctgaagcaggaatcgtctccaagtcatggatcccatccttatcgatgaccctcactgtgaagg
aaggcaggctcaggataaagcgcttctggagctctgctatacatttctttagtagttctactgcgtcctctcttgtcaggtctggtttgtagtagcgatc
cagaatactgagggttaggtaagccccgtagccatgtgcagcaaaacgcgtttttgctagagctgctaggtaatccatgtaatacaacgagggtccgtca
tgttcatcatacccagcaagcagcagattgacatggtaaggggtcctgctgcgaagatagtcagccagatttctccgtgtgaaagtttgcggctgcagta
ggagacaactcatagccgtttcctcattttgtacaactgcacgttcttttgaatgtactctgcaaactggactgtgtcacctgcctctcctacacacagc
agcaggatcttctcgctcatcttaaacatcttgtccacatcgtgcttcatcttaatgatgctgttggcacacactgtgtccgccagccccaattacaaaa
tcattgcccctgaatgccaatcaaatactccatattcccgccggctgttggttaccccgagttataagtctcctattacgggcctccgggactttacaca
gccaaatcagcacaaagcccggggatgggcgcc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: