XB-CLONE-4354532
Clone Name: IMAGE:7026732
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Gene:
atp6v1b2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782934
[+]
taattaatccccccccccccctggcagtctgaatcgctgagaagatggcggtgcgtgtaatgcggggaggtgagggcggcccggggaatcccataggtgc
agcggccagagaacatgtacaagcggtgagccgagactacctgtcccagcccaggctgacttatcgcactgtgtctggtgttaatgggcccctggttatc
ctggacaatgtcaagttccccaggtactcagagattgttcacctgactctccctgatggcacaaagagaagtgggcaggtactcgaggtcagcggatcca
aagctgtggtacaggtgtttgagggcacatccggcattgatgcaaagaaaacctcatgtgagttctctggggatattctcaggacaccagtatccgagga
catgttgggacgtgtatttaatggttcaggaaggcctattgatcgtggacccagtgttttagctgaggattatctagacataatggggcagcccataaac
ccccagtgcagaatctaccctgaagagatgatccagacagggatctctgctatagatggtatgaacagcattgcccgtggtcagaaaatccctattttct
ctgctgctgggcttccacataatgagattgcagctcagatctgtcgcccagcaggtctggtggaaaaatcccaagatgtaatgggattatagtggaggat
aactttggccatagttctttggctgctaatggggggttaaactgggaagaactccccgaatttctttcaagttctgaatattttgaaggaagacatggcc
ctcacttggaacaaaggggggggttcccctttttttgaaccctgtggcccattgaccccccccaatttgtagccgttatttaattaccccccaccgatcc
tttgggccccttttcccttctttggggaagattttcttgtggggcccatattataagtgggtgggaaaaaaaccccccctttacttgggc
3' EST: CF782933
[+]tttttttttttttttcaacattaaatgtaatatttactttgatttgacattcagcaataaatttatacaccgtatacagatggaatgaaccagacagcaa
actattgcaccaaattcacataaaataacacaaaacttccccccatagtaacagcctgcattctgagagactgggaagggcgtgccgaaataagaccaat
cttgaaataggaaggatgacggaaaatctaacatttcggagcaagtaaaaatggcagactgtgtgggagatgtaggtcctgcagggagttagataacaga
ggaaaaagcaaataaacagaaagggagggggggggagaatatccaaaggaacgcaggaggaatctggtaccaaagacaattgcctccctgcactcttcta
ccagtttctggtgcctatgggtgggcaaagggacaggcatggagtcaggcagcaggaaacagtgctggaaatggcaaaagccctgggcatcactataacc
aaccggtacatgcgcagggctaaattcctataaacacatgtgcttccagggctttttgcctagtttgtaattagggatttgccacgataatgtcgcttgt
gggtttctagaatttagtatcaactgcccccggtaaaatcacaggcactgctacacaataaatatacgcatgccacacaaggggaacatagagcacttca
tcttgcaccacattagcgtacaaaaccgcgggctactggaatcatattgnaaatgcttaaaatttgacatgaaatgaaatccacaggtcaatgggataga
a
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: