XB-CLONE-4354580
Clone Name: IMAGE:7026754
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Gene:
prkar2a
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF782904
[+]
taattaatcccccccccccccttttgttccgtgtttgagagattctgccatatataagaacttggaaagtgttgcattttaatagctcttcggatagcca
caaagatgtttcaacaataataggtctgagtattaatttgtaaaatgttttcgcatagctgggcttccctccctcacccccacagttggcctgctcagtc
ggaactctcgctcccttagccgttccgtatgatttaggttcacctagtagcaaaaatgttatgagacaaaaaaaaaaataaatcctgtatagtcatttta
gaacattttttatctttgtatctaagaagagaatgttaacacaagttcctattgtggctcctatggatttcctttccccccccccccaataggatatttt
gttattgagatcagtcaagcaccattcctaaaatattattcgaatacaaatgacatccacactgataaactctatatatctatatagtctttctcacaca
cactttgcactcccatttttattcaggagcaacattttaatgccttttttttttccctttttacccccccttgaatgtatgttaagaacaatggcaagac
tttctgcagcttgagtggagcgttccttacgttaggagaaagtaatacagtatgtcgtgcaacatgaaccaaaatgattattgtattcaatgaagcgttt
gtagcgtttctcaagcactccaagaaaacctccgaacataaattctaaagtacttggtn
3' EST: CF782903
[+]tttttttttttttttctttttttttttttttttttttttttttttttttttacaagtaacttaaaattttatgttcggaggtttcttggagtgcttgaaa
aacgctacaaacgcttcattgaatacaataatcattttggttcatgttgcacgacatactgtattactttctcctaacgtaaggaacgctccactcaagc
tgcaaaaagtcttgccattgttcttaacatacattcaagggggggtaaaaagggaaaaaaaaaaggcattaaaatgttgctcctgaataaaaatgggagt
gcaaagtgtgtgtgaaaaagactatatagatatatagagtttatcagtggggatgtcatttgtattccaataatattttaggaatggggcttgactgatc
tcaataacaaaatatcctattggggggggggggaaaggaaatccataggagccccaataggaacttgtgttaacattctcttcttagatacaaagataaa
aaatgttctaaaatgactatacaggatttatttttttttttggcccaaaacatttttgccaccagggggaacctaaatcatacggaaacgctaaggggag
ccagagttcccactgacccggccaacttttggggttaaggaaaggaaaccccactttgccaaaaaattttttaaaattaaaaccccgaacccttttattg
gttaaacaacttttgtggctttccccaaaaagctttttaaaaaggcgaccccttttccaagttctttaaattggggaaaaaattctccaacaacgggaac
aacaaagg
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: