XB-CLONE-4355081
Clone Name: IMAGE:7030497
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Gene:
c2h1orf74
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC082737
[+]
CAGAGCTGCGATTCATGTGATCTATGCAGGGAAGAGAGCTGGAAGATTTGAATGGAGAAGTGCTATGGCGCAGGATTTAAATGGCAAAAGAGTGCAGTGT
GTAGTACTACAGGGGGAAGGTTTTGCTTAAGGGAGAAAATTTACAGAAAAAAACACTGAACATCAGCCATTGAGACATCTTTCTCAATAATGGCCTCATC
CCTCCACAAACACTTGCTTTCAGCAGCTAGACACCACCTTAAGGAGACAAAGAGAATGTCAATGATGGTTGCTCTTAATCTTGCTGCTGAAATTCTGGCC
GTGGACTGCGGCCTGAAGCCTTGCTTTCTTTATGACTATACCACATCAGGAGTGCAGCAGATCTGTAGTTACCTCAAGGAACTTCAGAATCTAGGCTTAA
TTGTGGGCCATCTGCATATACTCAATGTTGAAGAAACTATCCTTATCATTAATGTAACAAAGGCAGTGTCTTACTTAGAAACATTGCTGCACAGCCAAGA
TTTACATCTAATTGATGTTTCTAATTATCTCAGTCAGCCAGAGTTGGTCAGCTCAAATCAAGTCCCTCAGATACATGCTCAACTTGCTGAGTTACTTGGT
CATATAAAACCTTACCAGAGCGGGCAGCCAACCTCAGTATCTGTAGGGGGTATCCAGTCGCCAGAGTGGAATCTTTGCACCATGTTTGGTTTTCTTCTTC
AGTTTCCTTCAACTTATTGGTTTGACACACAAAAAGGCTTTGAAAACTGCTTGTCATTTACACCCTTGAGGCTCTTCACTGTTCAAGCCAACTGTTCAAG
AATAGGCCACCAGAGTGTACAAATATATTCCTTTACTGTTCCTGAGTGTGTTTACCAAGCCACGCAAGTGCATTTGGAGGACTGGAGCAAAAGTCTAAAA
CAGGCATTTAATGAACAAAATTATTTCACAGACCTTGAAATAATTACAAATACCGCACAAGTATGCTGCGTATATTGACACAGCCGATTGTTGGAATCCT
GCAGCTACAGTCACAGTGAGGATCACATTTTCAGAAACAAATTGCGCTATAATCTGATCACAGAAAGTACTGATCTCATTAAATCAAATATAGTGTTTTT
TTACAATAATTTGCTACTTTTGGATTTACAGTCTACCAACCTTAGTATTCTCAAGATTTTTTTCTGCAGAGAATTTGTCTTCTGTTAATTTCTGGAGAGA
CTCTTTGGCCTTTTCTTCATACCTCTGCTTCTCTTCCTCCATCTCTAACAAGACTTTTTTCACACTTGTCTTTGGAAAATTCTGTAAAACATACACTTTT
GTTATGCTGTATGTATATAATGGTAAAACGAATGAACTTGTGTGTCTCTTCTAAAACATGAAAAGTTCAATGGATACTGTCATCTAGTTGCAAAACACTG
TAGTGATTATTCCTTAAAGTGGACCTGTCACCCAGACATGAAAATCTGTATAATAAAAGTCCTGTTCAAATTAAAAAAAAAAAAAAAA
5' EST: CF782644
[+]taattaatccccccccccccccagagctgcgattcatgtgatctatgcagggaagagagctggaagatttgaatggagaagtgctatggcgcaggattta
aatggcaaaagagtgcagtgtgtagtactacagggggaaggttttgcttaagggagaaaatttacagaaaaaaacactgaacatcagccattgagacatc
tttctcaataatggcctcatccctccacaaacacttgctttcagcagctagacaccaccttaaggagacaaagagaatgtcaatgatggttgctcttaat
cttgctgctgaaattctggccgtggactgcggcctgaagccttgctttctttatgactataccacatcaggagtgcagcagatctgtagttacctcaagg
aacttcagaatctaggcttaattgtgggccatctgcatatactcaatgttgaagaaactatccttatcattaatgtaacaaaggcagtgtcttacttaga
aacattgctgcacagccaagatttacatctaattgatgtttctaattatctcagtcagccagagttggtcagctcaaatcaagtccctcagatacatgct
caacttgctgagttacttggtcatataaaaccttaccagagcgggcagccaacctcagtatctgtagggggtatccagtcgccagagtggaatctttgca
ccatgtttggttttcttcttcagttcccttcaacttattggtttgacacacaaaaaggctttgaaaactgcttgtcatttacacccttgaggctcttcac
tggttcaagccaactgttcaagaataaggcaccagaagtgtacaaataatattcttt
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: