XB-CLONE-4355291
Clone Name: IMAGE:7030619
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Gene:
wdr74
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC077693
[+]
ACTCTAAGCAAGTATGGCAGCGCCTGCTCGGAATCACGTGTGGGTAGGAACCGAGACTGGAATATTGAAAGGCATTAATCTTCAAAAGAGACAAGCCATT
AATTACACAGATGTGGGATCCCTAACTAAGGGGCAGGAGGTTACTGCCATGTGCTGGGGTGATCCACAAGAGTCTGAGGTTTTTCTCGGTTGTGGAGACG
GCACGGTCAGAGTTTTCAGCAATGAAAAATCCAAATTCACTGAAATTCATGAGTGCAGAGGAGGGGAAGGGACATTTAAAGGACTTGCTGTTGTCGATAA
TGCTCTCGTAACGTGTGTGGAGTCTGGACTCTTAAAAGTTTGGAAGGCTGGGGAGTTTGATAGTCTAGAGGTGCAGGTTGGAGCTGGGATTGAGAAGATG
CGGCAGTGTGAAACCCAAAATCAGCGATTTGGAACAGGAGGCAAAGAGAATGACCTAAAAATCTGGGATTTGGAAAGACCTGAGGAGCCCGTTTTCAGAG
CTAAAAATTTAAGGAACGACTGGCTGGATCTCCGTGTACCTGTATGGATAAAGGATCTTGGATTTATTCCAGGGTCAGAGAAAATTGTAACATGCACAGG
TCATCACCAGGTCAGAATATATGATCCAAGCAGCCCCCAGAGACGTCCAGTCTTAGAGGTTCTGTATGAAGAAGATCCACTAACTGCGCTGAGTATCACC
CCAGATGGCAGGTCTGTCATTGTTGGAAATTCCCATGGGAATATGGCAGTTATCGATCTAAGGAAAGGCCGATTGCTTTGTGCTCTCAAGGGAAGTGCTG
GATCCATACGCTCCATCCAGTGCCACTCATCCATGCCTATTGTGGCTTCCTGTGGGCTTGACAGGTTCCTTCGAATCCACAACTATGAGAACAAAGAACT
GCAGCACAAGGTGTATCTGAAGTCTCGGTTAAACTGCCTTCTCTTAACGTCACGTGACAAATGGGAGGAAGATGATGTAACTTCAGCTGCAGAGCAGGAT
GTTAAGGAGGAGGAAGATGAAGTGTGGGACAACATGGAGACAGTGGTGGACAAAACCAGCAAAAAACGCCAGGGGGTTCAAATTAGGGCGCTGGAGGCTG
GAACTAAAAACCCCACCCGTAAAAAAAAGAAATGTGCTTGAGCTCTACTTTTTTTGGAGAAATGTATTGATAAAGTATAGGCAAGTACAAACATGACTAA
CTGCTCCGATGTCCAGACTGCCATCTCTGCTGCTGCATTCTCCTTTAATTCCATCTCAAGGAAAGAGATCTTTTCTGCAGTTCATGGAGCAGCTTTTGGA
TAAACTGAACAACAGCTGACTGATATAGTGAGGATGCTGGACCCCATGCAGATGAACCTTATTAACCTGTATTTTATATCTCATAGACTAAAGTGAAGTG
CTATCTATTGGATTTCATGTCACATTCATGTAAATATTTGTTCAATATAGGAATAAATAGATCCTTCCTACAAAAAAAAAAAAAAAA
5' EST: CF782539
[+]taattaatcccccccccccccactctaagcaagtatggcagcgcctgctcggaatcacgtgtgggtaggaaccgagactggaatattgaaaggcattaat
cttcaaaagagacaagccattaattacacagatgtgggatccctaactaaggggcaggaggttactgccatgtgctggggtgatccacaagagtctgagg
tttttctcggttgtggagacggcacggtcagagttttcagcaatgaaaaatccaaattcactgaaattcatgagtgcagaggaggggaagggacatttaa
aggacttgctgttgtcgataatgctctcgtaacgtgtgtggagtctggactcttaaaagtttggaaggctggggagtttgatagtctagaggtgcaggtt
ggagctgggattgagaagatgcggcagtgtgaaacccaaaatcagcgatttggaacaggaggcaaagagaatgacctaaaaatctgggatttggaaagac
ctgaggagcccgttttcagagctaanaatttaaggaacgactggctggatctccgtgtacctgtatggataaaggatcttggatttatttccggggtcaa
agaaaattgtaacatgcacaggtcatcaccaaggtcagaatataggatcccagccaccccccagaagacttccgggtcttaaagggttctggtatggaaa
aaaatccccttaacctggcgcttgaataaccaccccccaaaatgggcagggcccggcaattgggttgggaaatttc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | ![Expression Image Expression Image](image/thumbnail/XB-IMG-41423.jpg) |
Usage:
Suppliers: