XB-CLONE-4356451
Clone Name: IMAGE:7027786
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Gene:
srp19
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084521 [+]
GATCTTGCTGTGCGGAAACGCGAGTCAGCAAGGGCCGTGGGGCGTAATGAGAAGGGCTGTGCGGTAGCTGTACCCAGAGGAGAGCGGGCTGTTCAGACCT
TTACAACATAATAAAGAATGGCGAACTTGGAGAAATCCCATGCAAGTGTTGACAGGTTCATCTGCATTTATCCAGCTTACTTAAACAGTAAGAAAACTAT
TGCTGAGGGAAGGCGCATTCCCATAGAAAAGGCTGTGCAGAACCCGACATGTTCTGAGATTGCAGATATCTGTCGTGCCAACAAGCTGAATGCAGTTGTG
GAGGGGGATAAAATGTACACTCGGGAGTGGAACAGAGATACACAATTTAGGGGCAGAGTGCGGGTGCAGCTTAGGAACGAAGATGGAAGCAGTTGTGTCG
ATAAACTTTCATCAAGGAAAGCCATAATGCTGCGAGTAGCGGAAGAAATTCCAAAGCTGAAGACCAGAACACAGAAGAGTGGAGGTGGAGACCAAAGTGC
GCAACAGGGCGAAGGTGGCAAGAAGAACAAAAAGAAGAAGAAATGATTTGTTTTCGCCTGAAATAGACATTGTAAGGAGTAGGAGTGGTGCTAGACTCAA
GGGGAACTTAACGCTCCATTTCTGGAAAGTAACGTTTCAGACCTCTGAAGTGCTTAGTAAATACAGCTCTGTTTGATTATATAGGTTCATTGGCACAAAA
CTCCTGTTTGTTTTCCCAAGAACTCTTTTGAAAGTAACGGTAATGTAATGTTCATCTCCCCTATAATAAAGCAGCAAAGAGGCAATCATTTGAACTCCAT
CTGTGGCCTTTGTTTTATTGGAGCAAGCAAGGCCTTGGAGTACACCATGGAAATGCTGTTTTTTCTTCTGCCTATAATTCAAGATTGTTTTATTTAATTT
TTTTTTTTTTTTGAGCAAAGGCCAATGGGTCTATCAGTTGACCATGTACCCAGTTTTACAAAGGAATAAACAATGATTGGAAATGTGAAAAAAAAAAAAA
AAA
5' EST: CF781959 [+]taattaatcccccccccccccgatcttgctgtgcggaaacgcgagtcagcaagggccgtggggcgtaatgagaagggctgtgcggtagctgtacccagag
gagagcgggctgttcagacctttacaacataataaagaatggcgaacttggagaaatcccatgcaagtgttgacaggttcatctgcatttatccagctta
cttaaacagtaagaaaactattgctgagggaaggcgcattcccatagaaaaggctgtgcagaacccgacatgttctgagattgcagatatctgtcgtgcc
aacaagctgaatgcagttgtggagggggataaaatgtacactcgggagtggaacagagatacacaatttacgggcagagtgcgggtgcagcttacgaacg
aagatggaagcagttgtgtcgataaactttcatcaaggaaagccataatgctgcgagtagcggaagaaattccaaagctgaagaccagaacacaggaaga
gtgggaggtggagaccaaagtgcgcaacaggggcgaacgtggcaagaagaacaaaaagaagaagaaatgatttgttttcgcctgaaatagacattgtaag
gaagttagggagtgggtgcttagactccagggggaaatttaacgcctccccattttttttttgggggggggggggggggn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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