XB-CLONE-4356729
Clone Name: IMAGE:7029522
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Gene:
thrap3
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC080157 [+]
CCCGTTTAGCTTTGAGGCAGGAGTCAAGGAGCTGGCTTCTAAAGGGTTTGCGGGACGTTTAAGCAGCGATCCGGGACTGTGGCATTTGAAGCAGCACTAT
CGGAATCATTTGACTTGTTATTGCCTGAAAATATTGCCAAGATAACAGCGATCGTAAAAACATCACTGCTATAGGCAGGACTATTCAACTATTTAAAGAT
CCACAAGTCAGAATAAGACCTGACGGCTTGAGTTAAAAATGTCAAAAAACAAGTCAAAGTCCAGGTCAATATCTTCCCGCTCCATGTCCAGATCACGCTC
CCGATCCTTCTCAAAGTCTCGTTCCCGTTCAAGGTCTCTGTCACACTCGAGAAAACGCAGACACAGTTCTAGGTCTCGCTCAAGATCTTACTCTCCTAGT
TATAACAGAGAGCGGAATCACCCTAGAGTTTACCAAAACCGGGATTTCCGTGGCCACAACAGAGGATACCGGAGGCCATACTATTTTAGAGGCCGAGGAA
GAGGGTTTTACCCGAGAGGCCAGCATAACCGTGGGGGGTATGGAAACTATCGCCCTAACTGGCAGAATTACAACCGTCAACCCTACAGCCCACGCAGAGG
TCGATCTCGATCCCGCTCACCAAAGCGGAGATCTGGATCTCCAAGGTCACGGAGTCGTTCCAGGAATTCAGACAAATCCTCCTCAGATCGATCAAGGTCT
TCTTCATCAAGATCTTCTTCCAACCATAGCAGGGTGGAAGTTTCTAAGCGCAAGTCTAAGAAGGAGAAAAAAAGTCATTCCAAGGACCAGAGAGGGTCTG
CACAGGCTGTAGATGATGACTCAAAAGAACAGTCTGGATCTGGAGGACCAGACACAAGCAGCAAAACAGAGGGGTCCAAGGCATGGCAAGATATGGAAGC
TTATGATACAAGCCCAGTTCCACAAGATAGCCCTGCAGAGCGTGCCCCAACAGTCAAAAGCTCTGTCCAGTCTGTGGTGGTTAGGCGTTGCTCACCAAGA
CCTAGCCCTGTACAGAAGCCCAGTCCACCCTCCCCTTCTGTCCTGCAGGGCAGTGGAGCATATAGGGCTTCTCGCCAGAGTCCTTTTGATCATAGTTTGA
GCCCCCCACGAAAGAGTCCTCTTACTAAGAGCCCACCATTGGGTGCTTTATATGGTAATCAAAAGGAAGAGGGCAGGTCTGGAGAGATGTCAGCACCAAT
AGGAACAGGTTATAAAAGGTTTATGGAAGAACAGAAAAATAAGGCTGCTGAACTAGAGAAAAAAAAAAAAAAAA
5' EST: CF781820 [+]taattaatccccccccccccccccgtttagctttgaggcaggagtcaaggagctggcttctaaagggtttgcgggacgtttaagcagcgatccgggactg
tggcatttgaagcagcactatcggaatcatttgacttgttattgcctgaaaatattgccaagataacagcgatcgtaaaaacatcactgctataggcagg
actattcaactatttaaagatccacaagtcagaataagacctgacggcttgagttaaaaatgtcaaaaaacaagtcaaagtccaggtcaatatcttcccg
ctccatgtccagatcacgctcccgatccttctcaaagtctcgttcccgttcaaggtctctgtcacactcgagaaaacgcagacacagttctaggtctcgc
tcaagatcttactctcctagttataacagagagcggaatcaccctagagtttaccaaaaccgggatttccgtggccacaacagaggataccggaggccat
actattttagaggccgaggaagagggttttacccgagaggccagcataaccgtggggggtatggaaactatcgccctaactggcagaattacaaccgtca
accctacagcccacgcagaggtcgatctcgatcccgctcacaaagcggagatctggatctccaagtcacggagtccttccaggaattcagaacaatcctc
cctcgatcgatcaaggtcttcttcctcaagatcttcttccaacataacagggggggaaatttcctaccgccagttcctaaaaggagaaaaaaagtcattc
ccagggacgc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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