XB-CLONE-4356841
Clone Name: IMAGE:7029596
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Gene:
basp1
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Species: Xenopus tropicalis
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Sequence:
Full length sequence: BC084528 [+]
ACTAGAGCCCCAGCCACCGAGCAAGGGAGCCACAGCCACCCAGACTAAGCAGGAAACGTTGTGACACCAGGGCACACAGACACACACGGACAGACTCACA
CAACTCAGCAAGGGTCTGAAACATGGGAGGAAAGCTGAGCAAGAAGAAGAAGGGATACAATGTTAATGATGACAAAACTAAGGATAAAGACAAAAAGGCA
GAAGGAGCTAACACAGAAGAAGAAGGGGCACCTAAAACCAATGAAGAGACTCAGCCTGCTGTAGATAGTACAGAAGTAAAAGAAAATGATGCAAAGAATG
ATAAGGATGCACAGGCAACGGAAAGCAAAACAGAAAATAAAGAAGGGGAAAAAGAGGCACCAAACAAAGATGTTGTTCAGAAACCAGAGTCAGAGAAAAC
GGAAGCTTCTTCTGATACTAAAGCAGAGCCACAGAAAACAGAACAACCAGTATCAAAGCCAGAGGATCAACCAGCAGCAGCCCCTGCCCCTGTTACTAAT
AATGAAGGGCCAAAAGCCTCTGAGCCTAGTACTGAGGCCAAAGTTTCTCAGCCTTCAGAAGCACCAGCACCAAGTAAAGTAGAGGAAAAGAGTAAAGAGG
AAGGGGAAGCCAAAAAGACTGAGGCTCCCGCTTCATCAGAAATCAAAAGTGAGTCCGCCCCAGCTTCAGACTCAAAACCTAGCAGTGAGGCCGCACCTTC
TTCTGTGAAGTCTCAAGAAACTGAAGCACCTAGTTCTACTACTACTAAGGCCTCTGAGCCTGCAGGACCAGCTGATGAAGTTAAAGCTTCGGAGACCCCA
GCAGCTAATTCTGATCAAACCATAGCAGTTCAAGAGTAAAATGGACAGCCTGTTTGAAAATACCCATTAAAACAACCTGCGATGATGTTTTCTTCTTTGT
TTTTGATTATTTTTTTTCAGCTATACTAACAAACTTGATTTAGATCTGAAATAAGTACATGTAATGCCCAGAAAGGATGTTGAGATCCAAATAGTATTTT
TGAGGGAAAATGTACAAAATACACATTCCACCAACTATGTTAATTAATGAGTAACTTAAAGCTGTGGAAGTTTAGCGAGATAGATGTTTGCAAGGAAAAA
AAATGAATCTTTTACATGTAAGCAGCAGATTCAAAGAATAAGTAGCCAAAATAACACATTTTGTTTCTTGCTTCTGCAGTAAAAATGCAGGAGAAATGTT
CTTGAATTTGCCCGTAGGGCTTGTGCCAAGGCAATCAAACCTTTTAAAGAATTTTTTTTTATTTACAGTCGATATATTGTTTTTTATTTTTTTTCAAATG
CAATGAATAATGGCCAAACTTTTTTCAAAAGAACCCACTTAGCTGTCTCTTATTCCTCTTCAGATATTTTGGGAATAATAAACATCCTCATCCTACTTAG
TCTACCTAGATTTCTCATGATGAATTAATGCATGTCTGTGGTTGGGTGCACCTGTAGTTCTGTTTATTGGTCAGTGGAAATAAAAAAAAAAGTCTGCGTT
GAAAAAAAAAAAAAAAA
5' EST: CF781764 [+]taattaatcccccccccccccactagagccccagccaccgagcaagggagccacagccacccagactaagcaggaaacgttgtgacaccagggcacacag
acacacacggacagactcacacaactcagcaagggtctgaaacatgggaggaaagctgagcaagaagaagaagggatacaatgttaatgatgacaaaact
aaggataaagacaaaaaggcagaaggagctaacacagaagaagaaggggcacctaaaaccaatgaagagactcagcctgctgtagatagtacagaagtaa
aagaaaatgatgcaaagaatgataaggatgcacaggcaacggaaagcaaaacagaaaataaagaaggggaaaaagaggcaccaaacaaagatgttgttca
gaaaccagagtcagagaaaacggaagcttcttctgatactaaagcagagccacagaaaacagaacaaccagtatcaaagccagaggatcaaccagcagca
gcccctgcccctgttactaataatgaaggggccaaagcctctgagcctagtactgaggccaaagtttctcagccttcagaagcaccagcaccaagtaaag
tagaggaaaagaataaagaggaaggggaagccaaaaagactgaggctccccgcttcatcagaaaatcaaaagtgagttcgccccagctttcagactcaaa
acctaaccattgaaggccgccaccttctttctgtgaaagtcctcagaaaacggaaagccccctagtttctacctactaacaaagggc
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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