XB-CLONE-4357991
Clone Name: IMAGE:7028334
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Gene:
rpl18
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF781189
[+]
taattaatccccccccccccccgccctttcctccccgagaagccgctgctgctacagccgccatcatgggagtagatatccgtcacaacaaggaccgcaa
ggtgcggcgaaaggagcccaagagccaggatatcaaactgaggcttttactaaagctttatcgtttcatggccatacgtaccaactcaagtttcaacagg
gtggttctgaagcgtctgtccatgagccgaaccaacagggctcccctctctgtgtctcgtattattctcataatgaaattgacagggcgggaaaaagagg
attgaagtggtgggagggatgtattagagaggaggtacatgatctaaagaaatactaaagcggttgccaagcgcggttgggaggttggacggaggtctcg
atattgtttcaagtacatgcttaacagtggtttaactagaatagagcgcacgagcttgcgcggaatatatgttcgtagtaaaagggatttattggtcgtg
gaggagatttcgaggaatggaggggtaagaggggacgatggacttctgtaattgcatagatatattggagggcatggtaggagggtccggaagatattgt
tgactttcgctgttgtccagcagttcgcggctgggttgagtgaacgcaataacctcatactgcgcaagacaaataagaccaactcttctgagatgatgaa
cagtctaaacacgattcatagagtggagagttcgcagttggagtctagcgacacactacacgtattgtttggtgggtgaagataaaggggcgacgtatgg
ttccncaagtcaacaagaanagctcatgagatgttaaagataagaaatacagcggtaaagaaaacaaaccctgcacgcanataggagtagtgagattcgt
gggnngtgcgagggtagagtcagacatagaaatgatgaataagaaatgatattagataattggacggtggaagagaagaaagaaaatttgtntatgggga
ttctactgaggaatataatatctagggagnaatatgcacaataaggtaaacttgantgtattgactctggtagcaagtataatatggggcggttcttcaa
gcataaatagaggtatgaggatgtgtatggaagataatgaatgaaagggaagtgaaagaatggattatatgtgcgtagtttangataatctgaaggatat
agaggtgggttttttgtgggttgtaaanatggcncgagagtttttataggattttatgataatagtaaattttgaaagtaatcagatataagaagatgct
gctcataagatgcgggtgtgtagagggggtaggttgggaaaagaggattaaggtaaggtgggggttacgtgatgcgctcgtaaagatnaaggaagaagag
gattaaaatgaatgaatgccgggtacgctcgtctggctgcttatatggatgcacattntaacatagttgaggcatagcaaaagaggaaaacagcgaggta
gcag
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI |  |
Usage:
Suppliers: