XB-CLONE-4352475
Clone Name: IMAGE:7027411
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Gene:
ocm4.2
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Species: Xenopus tropicalis
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Sequence:
5' EST: CF784226 [+]
taattaatcccccccccccccgacacatctgtcacaggaagactgcttgtatctcttcactccagctacagaggacttcggactgaccatggcattcggt
ggtatcctgagtgatgctgacgtctctgctgctctgcagaactgccaagctcctgaatccttcaacttcaaaactttctttgcccagtctggcctgagca
gcaagtccgacaatgatgtgaggaacgtctttgccatccttgaccaggacaggagcggcttcattgaggaagatgaactgaaactgttcctccagaactt
caacgcaggtgccagggctttaactgatgctgagaccaaggccttcctgaatgctggtgactctgatggtgatggcaaaattggagttgatgagttccag
gctttggtgaaaaactaattttttaaaaaactggaatatgcgaaacatcagaaaagatgaatttggttttctgcattaaactgatccaagtgaaacctgg
tttagcggaacacagatgcctcttcaatatttctcctcatgagaaaagttgtgatgtcatcctgcggtggtgggatccttttactttttcaccccagaac
catggttacccttgtctaccgtttatcccggggcccccttcgttaaaccaaaatacctatcctcatccttttaaccccctccatcctcccctattgccct
acccctgttttcagacttttttacaaatatctcgtcccacctcgttaattttctccggttttacccctcccccggtggtgtaatttccgccccggcgccc
caccacttttttatccacctcgcgcccccttctattaaataacgccctcccatccgtggataattccac
3' EST: CF784225 [+]tttttttttttttttcgattccaaaaatgatttattgagagcaatttgtttcttgtatgcctgcctcagtccacaggaaactgaagcaagaattgcaagt
tcatggatttgtaaaagtctcaacagtgtaaacaattgacaacgtgattagtataaaaatgagaataaatatgttggtttaacgaaggggcccttggata
aactgtagacaagggtaaccatgcttctctggtgaattaataaatggatcccaccaccgcatgatgacatcacaacttttctcatgaggagaaatattga
agaggcatctgtgttccgctaaaccaggtttcacttggatcagtttaatgcagaaaaccaaattcatcttttctgatgtttcgcatattccagtttttta
aaaaattagtttttcaccaaagcctggaactcatcaactccaattttgccatcaccatcagagtcaccagcattcaggaaggccttggtctcagcatcag
ttaaagccctggcacctgcgttgaagttctggaggaacagtttcagttcatcttcctcaatgaagccgctcctgtcctggtcaaggatggcaaagacgtt
cctcacatcattgtcggacttgctgctcaggccagactgggcaaagaaagttttgaagttgaaggattcaggagcttggcagttctgcagagcagcagag
acgtcagcatcactcaggataccaccgaatgccatggtcagtccgaagtcctctgtagctggagtgaagagaacaagcagtcttcctgtgacaatgttcn
ggn
Library:
Description: 10 ug of polyA+ RNA was isolated from a mixture of embryos at stage 10, 20 and 30 and primed by oligo-dT primer: 5'-GAGAGAGAGAAGGATCC(T)16VN-3' (where V=G,A,C). 5-methyl-dCTP was used instead of dCTP in the first-strand synthesis in order to get hemimethylated cDNA. After full-length enrichment, oligo-dG tailing and normalization against itself, second-strand synthesis was carried out by priming with 5'-GAGAGAGAGACTCGAGTTAATTAAT(C)13-3'. dsDNA was digested with XhoI/BamHI and directionally cloned into the pRKW2 vector. Average insert size is 1.5 kb. Library constructed using the Carninci protocol (Genome Research 2000) by Drs. W. Wu and C. Niehrs (DKFZ, Heidelberg, Germany). Vector Details | Vector Map |
Name: pRKW2
Type: phagemid
5' Restriction Site: XhoI
3' Restriction Site: BamHI | |
Usage:
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