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XB-LIB-32
Library Name: NIH_XGC_tropBrn4 |
Species: Xenopus tropicalis |
Number Of Clones: 11223 |
Description: PolyA+ RNA was primed with oligo-dT primer 5'-GACTAGTTCTAGATCGCGAG CGGCCGCCCTTTTTTTTTTTTTTT-3'. cDNA was ligated to SalI adapter (5'-TCGACCCACGCGTCCG-3' and 5'-CGGACGCGTGGG-3'), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into SalI and NotI digested pCMV-SPORT6 vector. Library contains the 2-3 kb size fraction (additional size fractions contained in the NIH_XGC_tropBrn2 and Brn3 libraries). Library constructed at the DOE Joint Genome Institute.
| Normalized: No | Subtraction: No |
| Anatomy: brain | Stage: frog |
| Vector Details | Vector Map | Name: pCMV-SPORT6 Type: phagemid 5' Restriction Site: SalI 3' Restriction Site: NotI |  |