Return to search results Title: The identification of differentially expressed genes between animal and vegetal blastomeres in Xenopus laevis

Summary

To identify asymmetrically localized maternal mRNAs along the animal-vegetal axis in cleavage Xenopus embryos, we isolated animal and vegetal blastomeres at 8-cell stage, extracted the maternal mRNA respectively and analyzed them by RNA-seq technology. We identified 43 maternal transcripts significantly enriched in the vegetal region (FDR<0.05) by R/Bioconductor package DESeq

Contributors: Xiaopeng Ma, Guanni Sun, Zhirui Hu, Zheying Min, Xiaohua Yan, Zhenpo Guan, Hanxia Su, Yu Fu, YeGuang Chen, Michael Zhang, Qinghua Tao, Wei Wu

Experiment Type: RNAseq of animal and vegetal blastomeres with 2 biological replicates

Article: XB-ART-50741, PubMed

Source: NCBI GEO, Xenbase Download

Sample	View		GSMs	Assay Type
animal blastomere - NF4 GSM1689111  GSM1689112  RNA-Seq Xla   [-] [+] Background: Xla.Outbred Conditions: Tissue Assay Stages ChIP Antibody animal blastomere NF stage 4 (8-cell)   vegetal blastomere - NF4 GSM1689109  GSM1689110  RNA-Seq Xla   [-] [+] Background: Xla.Outbred Conditions: Tissue Assay Stages ChIP Antibody vegetal blastomere NF stage 4 (8-cell)

Select All  Xtr    Xla

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