Return to search results Title: Detection of RNAs binding to Vg1 RNP

Summary

We report the application of NGS for high-throughput profiling of transcripts assoicated with the ribonuleoprotein (RNP) formed on Vg1 mRNA in oocytes of Xenopus Laevis.

Contributors: Chao Yang, Paul Huber

Experiment Type: Biriefly, Biotinylated-Vg1 RNA was microinjected into Xenopus oocyte to allow formation of RNP complex in vivo. Next, streptavidin beads were added to the oocyte extract to specificly pull down the biotin-Vg1 RNP. After extensive washes, biotin-Vg1 RNP were eluted off by incubating the beads with free biotin and the associted RNAs were extracted by phenol and precipitated by ethanol.For RNA sequencing strategy, a single RNA library was prepared and sequenced on an Illumina MiSeq nano 300 cycle reagent kit.

Experiment Reagents: gdf1, streptavidin-beads.

Source: NCBI GEO, Xenbase Download

Sample	View		GSMs	Assay Type
oocyte + biotin-gdf1 + streptavidin-beads - oocyte III-IV GSM4679353  RNA-Seq [-] [+] Background: Xla.Inbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area mRNA injection gdf1 (Xla) gdf1 (Xla) oocyte stage III oocyte peptide/protein streptavidin-beads oocyte stage III Conditions: Tissue Assay Stages ChIP Antibody oocyte oocyte stage III to oocyte stage IV

Select All  Xtr    Xla

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