Return to search results Title: Whole embryo depletion of Taf15 in gastrula and neurula stage Xenopus tropicalis embryos

Summary

We examined the role of Taf15 in in gastrula and neurula stage Xenopus tropicalis embryos.

Contributors: Richard Harland, Caitlin DeJong, Darwin Dichmann, Cameron Exner, Yuxiao Xu, Richard Harland

Experiment Type: To determine the role of Taf15 in early Xenopus development, we used RNA-sequencing (RNA-seq) from single embryos depleted of M- and Z-Taf15, using reagents that targeted either all mRNA by inhibiting translation with Morpholino antisense and mismatch oligonucleotides (MOs) or only zygotic function using splice-blocking MOs or CRISPR-mediated mutagenesis.

Experiment Reagents: taf15 MO1.

Source: NCBI GEO, Xenbase Download

Sample	View		GSMs	Assay Type
WE - NF10 GSM5467454  GSM5467455  GSM5467458  RNA-Seq Xla   [-] [+] Background: Xla.Inbred Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 10      WE + taf15 MO - NF10 DEG GSM5467456  GSM5467457  GSM5467459  RNA-Seq Xla   [-] [+] Background: Xla.Inbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area morpholino injection taf15 MO1 taf15 (Xla) NF stage 2 (2-cell) blastomere Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 10   WE - NF15 GSM5467460  GSM5467464  GSM5467465  GSM5467466  RNA-Seq Xla   [-] [+] Background: Xla.Inbred Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 15      WE + taf15 MO - NF15 DEG GSM5467461  GSM5467462  GSM5467463  RNA-Seq Xla   [-] [+] Background: Xla.Inbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area morpholino injection taf15 MO1 taf15 (Xla) NF stage 2 (2-cell) blastomere Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 15

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