Return to search results Title: Genome-wide binding pattern of β-catenin during Xenopus gastrulation

Summary

The canonical Wnt/β-catenin signaling pathway plays multiple roles during Xenopus gastrulation, including posteriorization of the neural plate, patterning of the mesoderm, and induction of the neural crest. Wnt signaling stabilizes β-catenin, which then activates target genes. However, few targets of this signaling pathway that mediate early developmental processes are known. Here we sought to identify transcriptional targets of the Wnt/β-catenin signaling pathway using a genome-wide approach.

Contributors: Richard Harland, Rachel Kjolby, Richard Harland

Experiment Type: Xenopus laevis emrbyos were injected with a triple FLAG tagged beta-catenin at levels that did not effect phenotype. Pools of about 500 embryos were fixed for ChIP processing. An input sample was taken prior to immunoprecipitation with a Flag antibody. Both input and ChIP material were used to make sequencing libraries.

Experiment Reagents: Tg(ctnnb1-FLAG).

Article: XB-ART-52028, PubMed

Source: NCBI GEO, Xenbase Download

Sample	View		GSMs	Assay Type
input WE - NF11.5 GSM2050741  GSM2050742  GSM2050743  ChIP-Seq [-] [+] Background: Xla.Outbred Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 11.5      beta Catenin WE - NF11.5 GSM2050738  GSM2050739  GSM2050740  ChIP-Seq Xla   [-] [+] Background: Xla.Outbred Manipulations: Manipulation Type Reagent Target Gene Stage Treatment Area mRNA injection Tg(ctnnb1-FLAG) ctnnb1.L NF stage 2 (2-cell) animal blastomere Conditions: Tissue Assay Stages ChIP Antibody embryo NF stage 11.5 FLAG Ab1

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Select All  Xtr    Xla

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