GEO Series: GSE77363
The canonical Wnt/β-catenin signaling pathway plays multiple roles during Xenopus gastrulation, including posteriorization of the neural plate, patterning of the mesoderm, and induction of the neural crest. Wnt signaling stabilizes β-catenin, which then activates target genes. However, few targets of this signaling pathway that mediate early developmental processes are known. Here we sought to identify transcriptional targets of the Wnt/β-catenin signaling pathway using a genome-wide approach.
Contributors: Richard Harland, Rachel Kjolby, Richard Harland
Experiment Type: Xenopus laevis emrbyos were injected with a triple FLAG tagged beta-catenin at levels that did not effect phenotype. Pools of about 500 embryos were fixed for ChIP processing. An input sample was taken prior to immunoprecipitation with a Flag antibody. Both input and ChIP material were used to make sequencing libraries.
Experiment Reagents: Tg(ctnnb1-FLAG).
Article: XB-ART-52028, PubMed
Source: NCBI GEO, Xenbase Download
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