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Anterior axis duplication in Xenopus induced by the over-expression of the cadherin-binding protein plakoglobin.
Karnovsky A
,
Klymkowsky MW
.
???displayArticle.abstract??? Plakoglobin interacts with both classical and desmosomal cadherins. It is closely related to Drosophila aramadillo (arm) gene product; arm acts in the wingless (wg)-signaling pathway to establish segment polarity. In Xenopus, homologs of wg--i.e., wnts, can produce anterior axis duplications by inducing dorsal mesoderm. Studies in Drosophila suggest that wnt acts by increasing the level of cytoplasmic armadillo protein (arm). To test whether simply increasing the level of plakoglobin mimics the effects of exogenous wnts in Xenopus, we injected fertilized eggs with RNA encoding an epitope-tagged form of plakoglobin; this induced both early radial gastrulation and anterior axis duplication. Exogenous plakoglobin accumulates in the nuclei of embryonic cells. Plakoglobin binds to the tail domain of the desmosomal cadherin desmoglein 1. When RNA encoding the tail domain of desmoglein was coinjected with plakoglobin RNA, both the dorsalizing effect and nuclear accumulation of plakoglobin were suppressed. Mutational analysis indicates that the central arm repeat region of plakoglobin is sufficient to induce axis duplication and that this polypeptide accumulates in the nuclei of embryonic cells. These data show that increased plakoglobin levels can, by themselves, generate the intracellular signals involved in the specification of dorsal mesoderm.
Bejarano,
Mutants shed light on plant development.
1992, Pubmed
Bejarano,
Mutants shed light on plant development.
1992,
Pubmed
Bradley,
Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion.
1993,
Pubmed
Burrus,
Wnt-1 as a short-range signaling molecule.
1994,
Pubmed
,
Xenbase
Cary,
Differential organization of desmin and vimentin in muscle is due to differences in their head domains.
1994,
Pubmed
,
Xenbase
Cary,
Vimentin's tail interacts with actin-containing structures in vivo.
1994,
Pubmed
,
Xenbase
Cowin,
Plakoglobin: a protein common to different kinds of intercellular adhering junctions.
1986,
Pubmed
DeMarais,
The armadillo homologs beta-catenin and plakoglobin are differentially expressed during early development of Xenopus laevis.
1992,
Pubmed
,
Xenbase
Evan,
Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product.
1985,
Pubmed
,
Xenbase
Fortini,
An activated Notch receptor blocks cell-fate commitment in the developing Drosophila eye.
1993,
Pubmed
Fouquet,
Identification of plakoglobin in oocytes and early embryos of Xenopus laevis: maternal expression of a gene encoding a junctional plaque protein.
1992,
Pubmed
,
Xenbase
Funayama,
Embryonic axis induction by the armadillo repeat domain of beta-catenin: evidence for intracellular signaling.
1995,
Pubmed
,
Xenbase
Heasman,
Overexpression of cadherins and underexpression of beta-catenin inhibit dorsal mesoderm induction in early Xenopus embryos.
1994,
Pubmed
,
Xenbase
Hinck,
Wnt-1 modulates cell-cell adhesion in mammalian cells by stabilizing beta-catenin binding to the cell adhesion protein cadherin.
1994,
Pubmed
Hülsken,
Tumor-suppressor gene products in cell contacts: the cadherin-APC-armadillo connection.
1994,
Pubmed
Kao,
The entire mesodermal mantle behaves as Spemann's organizer in dorsoanterior enhanced Xenopus laevis embryos.
1988,
Pubmed
,
Xenbase
Kapprell,
Biochemical characterization of the soluble form of the junctional plaque protein, plakoglobin, from different cell types.
1987,
Pubmed
Klymkowsky,
Evidence that the deep keratin filament systems of the Xenopus embryo act to ensure normal gastrulation.
1992,
Pubmed
,
Xenbase
Knudsen,
Plakoglobin, or an 83-kD homologue distinct from beta-catenin, interacts with E-cadherin and N-cadherin.
1992,
Pubmed
Kopan,
The intracellular domain of mouse Notch: a constitutively activated repressor of myogenesis directed at the basic helix-loop-helix region of MyoD.
1994,
Pubmed
Korman,
Demonstration of an adhering-junction molecule (plakoglobin) in the autoantigens of pemphigus foliaceus and pemphigus vulgaris.
1989,
Pubmed
Kowalczyk,
Posttranslational regulation of plakoglobin expression. Influence of the desmosomal cadherins on plakoglobin metabolic stability.
1994,
Pubmed
Ku,
Xwnt-11: a maternally expressed Xenopus wnt gene.
1993,
Pubmed
,
Xenbase
Legan,
The molecular biology of desmosomes and hemidesmosomes: "what's in a name"?
1992,
Pubmed
Lieber,
Antineurogenic phenotypes induced by truncated Notch proteins indicate a role in signal transduction and may point to a novel function for Notch in nuclei.
1993,
Pubmed
Mathur,
Interactions of the cytoplasmic domain of the desmosomal cadherin Dsg1 with plakoglobin.
1994,
Pubmed
McCrea,
Induction of a secondary body axis in Xenopus by antibodies to beta-catenin.
1993,
Pubmed
,
Xenbase
McMahon,
Ectopic expression of the proto-oncogene int-1 in Xenopus embryos leads to duplication of the embryonic axis.
1989,
Pubmed
,
Xenbase
Moon,
Dissecting Wnt signalling pathways and Wnt-sensitive developmental processes through transient misexpression analyses in embryos of Xenopus laevis.
1993,
Pubmed
,
Xenbase
Noordermeer,
dishevelled and armadillo act in the wingless signalling pathway in Drosophila.
1994,
Pubmed
Parkin,
Activity of Wnt-1 as a transmembrane protein.
1993,
Pubmed
,
Xenbase
Peifer,
The segment polarity gene armadillo encodes a functionally modular protein that is the Drosophila homolog of human plakoglobin.
1990,
Pubmed
Peifer,
The vertebrate adhesive junction proteins beta-catenin and plakoglobin and the Drosophila segment polarity gene armadillo form a multigene family with similar properties.
1992,
Pubmed
,
Xenbase
Peifer,
wingless signal and Zeste-white 3 kinase trigger opposing changes in the intracellular distribution of Armadillo.
1994,
Pubmed
Perrimon,
The genetic basis of patterned baldness in Drosophila.
1994,
Pubmed
Rupp,
Xenopus embryos regulate the nuclear localization of XMyoD.
1994,
Pubmed
,
Xenbase
Siegfried,
Components of wingless signalling in Drosophila.
1994,
Pubmed
Sive,
The frog prince-ss: a molecular formula for dorsoventral patterning in Xenopus.
1993,
Pubmed
,
Xenbase
Struhl,
Intrinsic activity of the Lin-12 and Notch intracellular domains in vivo.
1993,
Pubmed
Troyanovsky,
Identification of the plakoglobin-binding domain in desmoglein and its role in plaque assembly and intermediate filament anchorage.
1994,
Pubmed