Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
Embryonic axis induction by the armadillo repeat domain of beta-catenin: evidence for intracellular signaling.
Funayama N
,
Fagotto F
,
McCrea P
,
Gumbiner BM
.
???displayArticle.abstract???
beta-catenin was identified as a cytoplasmic cadherin-associated protein required for cadherin adhesive function (Nagafuchi, A., and M. Takeichi. 1989. Cell Regul. 1:37-44; Ozawa, M., H. Baribault, and R. Kemler. 1989. EMBO [Eur. Mol. Biol. Organ.] J. 8:1711-1717). Subsequently, it was found to be the vertebrate homologue of the Drosophila segment polarity gene product Armadillo (McCrea, P. D., C. W. Turck, and B. Gumbiner. 1991. Science [Wash. DC]. 254:1359-1361; Peifer, M., and E. Wieschaus. 1990. Cell. 63:1167-1178). Also, antibody perturbation experiments implicated beta-catenin in axial patterning of the early Xenopus embryo (McCrea, P. D., W. M. Brieher, and B. M. Gumbiner. 1993. J. Cell Biol. 123:477-484). Here we report that overexpression of beta-catenin in the ventral side of the early Xenopus embryo, by injection of synthetic beta-catenin mRNA, induces the formation of a complete secondary body axis. Furthermore, an analysis of beta-catenin deletion constructs demonstrates that the internal armadillo repeat region is both necessary and sufficient to induce axis duplication. This region interacts with C-cadherin and with the APC tumor suppressor protein, but not with alpha-catenin, that requires the amino-terminal region of beta-catenin to bind to the complex. Since alpha-catenin is required for cadherin-mediated adhesion, the armadillo repeat region alone probably cannot promote cell adhesion, making it unlikely that beta-catenin induces axis duplication by increasing cell adhesion. We propose, rather, that beta-catenin acts in this circumstance as an intracellular signaling molecule. Subcellular fractionation demonstrated that all of the beta-catenin constructs that contain the armadillo repeat domain were present in both the soluble cytosolic and the membrane fraction. Immunofluorescence staining confirmed the plasma membrane and cytoplasmic localization of the constructs containing the armadillo repeat region, but revealed that they also accumulate in the nucleus, especially the construct containing only the armadillo repeat domain. These findings and the beta-catenin protein interaction data offer several intriguing possibilities for the site of action or the protein targets of beta-catenin signaling activity.
Bradley,
Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion.
1993, Pubmed
Bradley,
Expression of Wnt-1 in PC12 cells results in modulation of plakoglobin and E-cadherin and increased cellular adhesion.
1993,
Pubmed
Breen,
Poorly differentiated colon carcinoma cell lines deficient in alpha-catenin expression express high levels of surface E-cadherin but lack Ca(2+)-dependent cell-cell adhesion.
1993,
Pubmed
Brieher,
Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
1994,
Pubmed
,
Xenbase
Choi,
A cadherin-like protein in eggs and cleaving embryos of Xenopus laevis is expressed in oocytes in response to progesterone.
1990,
Pubmed
,
Xenbase
Detrick,
The effects of N-cadherin misexpression on morphogenesis in Xenopus embryos.
1990,
Pubmed
,
Xenbase
Fagotto,
Beta-catenin localization during Xenopus embryogenesis: accumulation at tissue and somite boundaries.
1994,
Pubmed
,
Xenbase
Fujimori,
Ectopic expression of N-cadherin perturbs histogenesis in Xenopus embryos.
1990,
Pubmed
,
Xenbase
Gumbiner,
Catenins as mediators of the cytoplasmic functions of cadherins.
1993,
Pubmed
,
Xenbase
Hatzfeld,
Band 6 protein, a major constituent of desmosomes from stratified epithelia, is a novel member of the armadillo multigene family.
1994,
Pubmed
Heasman,
Overexpression of cadherins and underexpression of beta-catenin inhibit dorsal mesoderm induction in early Xenopus embryos.
1994,
Pubmed
,
Xenbase
Hinck,
Wnt-1 modulates cell-cell adhesion in mammalian cells by stabilizing beta-catenin binding to the cell adhesion protein cadherin.
1994,
Pubmed
Hirano,
Identification of a neural alpha-catenin as a key regulator of cadherin function and multicellular organization.
1992,
Pubmed
Levine,
Selective disruption of E-cadherin function in early Xenopus embryos by a dominant negative mutant.
1994,
Pubmed
,
Xenbase
Matsuyoshi,
Cadherin-mediated cell-cell adhesion is perturbed by v-src tyrosine phosphorylation in metastatic fibroblasts.
1992,
Pubmed
McCrea,
Purification of a 92-kDa cytoplasmic protein tightly associated with the cell-cell adhesion molecule E-cadherin (uvomorulin). Characterization and extractability of the protein complex from the cell cytostructure.
1991,
Pubmed
,
Xenbase
McCrea,
A homolog of the armadillo protein in Drosophila (plakoglobin) associated with E-cadherin.
1991,
Pubmed
,
Xenbase
McCrea,
Induction of a secondary body axis in Xenopus by antibodies to beta-catenin.
1993,
Pubmed
,
Xenbase
McMahon,
Ectopic expression of the proto-oncogene int-1 in Xenopus embryos leads to duplication of the embryonic axis.
1989,
Pubmed
,
Xenbase
Nagafuchi,
Transmembrane control of cadherin-mediated cell adhesion: a 94 kDa protein functionally associated with a specific region of the cytoplasmic domain of E-cadherin.
1989,
Pubmed
Nagafuchi,
Cell binding function of E-cadherin is regulated by the cytoplasmic domain.
1988,
Pubmed
Nagafuchi,
The roles of catenins in the cadherin-mediated cell adhesion: functional analysis of E-cadherin-alpha catenin fusion molecules.
1994,
Pubmed
Noordermeer,
dishevelled and armadillo act in the wingless signalling pathway in Drosophila.
1994,
Pubmed
Ozawa,
The cytoplasmic domain of the cell adhesion molecule uvomorulin associates with three independent proteins structurally related in different species.
1989,
Pubmed
Peifer,
The segment polarity gene armadillo encodes a functionally modular protein that is the Drosophila homolog of human plakoglobin.
1990,
Pubmed
Peifer,
The segment polarity gene armadillo interacts with the wingless signaling pathway in both embryonic and adult pattern formation.
1991,
Pubmed
Peifer,
A repeating amino acid motif shared by proteins with diverse cellular roles.
1994,
Pubmed
Peifer,
wingless signal and Zeste-white 3 kinase trigger opposing changes in the intracellular distribution of Armadillo.
1994,
Pubmed
Peifer,
A model system for cell adhesion and signal transduction in Drosophila.
1993,
Pubmed
Rubinfeld,
Association of the APC gene product with beta-catenin.
1993,
Pubmed
Shiozaki,
Immunohistochemical detection of alpha-catenin expression in human cancers.
1994,
Pubmed
Siegfried,
Components of wingless signalling in Drosophila.
1994,
Pubmed
Smith,
Expression cloning of noggin, a new dorsalizing factor localized to the Spemann organizer in Xenopus embryos.
1992,
Pubmed
,
Xenbase
Sokol,
Injected Wnt RNA induces a complete body axis in Xenopus embryos.
1991,
Pubmed
,
Xenbase
Su,
Association of the APC tumor suppressor protein with catenins.
1993,
Pubmed
Wilson,
The structure of an antigenic determinant in a protein.
1984,
Pubmed
van Leeuwen,
Biological activity of soluble wingless protein in cultured Drosophila imaginal disc cells.
1994,
Pubmed