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The ubiquitous nuclear protein, NHP1, binds with high affinity to different sequences of the chicken vitellogenin II gene.
Hughes MJ
,
Jost JP
.
Abstract
In gel shift assays, affinity chromatography-purified NHP1 forms a stable complex with different sequences of the chicken vitellogenin II gene. The apparent KD of NHP1 with the estrogen response element (ERE) containing 5-methylcytosine is 1 X 10(-11) M. NHP1 does not form a complex with the Xenopus vitellogenin ERE where the GCG bases are replaced by CAG. NHP1 is closely related if not identical to the other ubiquitous proteins NHP2, NHP3 and NHP4 that bind specifically to different sequences. All four proteins behave identically on chromatography and give identical patterns in proteolytic bandshift assays. NHP1, NHP2 and NHP3 have a native molecular weight of 170,000 and are composed of two polypeptides of 85 and 75 kDa. The possible function of NHP1 is discussed.
Burch,
Chromatin structural transitions and the phenomenon of vitellogenin gene memory in chickens.
1986, Pubmed
Burch,
Chromatin structural transitions and the phenomenon of vitellogenin gene memory in chickens.
1986,
Pubmed
Burch,
Two functional estrogen response elements are located upstream of the major chicken vitellogenin gene.
1988,
Pubmed
Burch,
Temporal order of chromatin structural changes associated with activation of the major chicken vitellogenin gene.
1983,
Pubmed
Davidson,
The HeLa cell protein TEF-1 binds specifically and cooperatively to two SV40 enhancer motifs of unrelated sequence.
1988,
Pubmed
Feavers,
Interaction of two nonhistone proteins with the estradiol response element of the avian vitellogenin gene modulates the binding of estradiol-receptor complex.
1987,
Pubmed
Geiser,
Structure and sequence of the promoter area and of a 5' upstream demethylation site of the estrogen-regulated chicken vitellogenin ii gene.
1983,
Pubmed
Germond,
Evolution of vitellogenin genes: comparative analysis of the nucleotide sequences downstream of the transcription initiation site of four Xenopus laevis and one chicken gene.
1984,
Pubmed
,
Xenbase
Jiricny,
A human 200-kDa protein binds selectively to DNA fragments containing G.T mismatches.
1988,
Pubmed
Jost,
Association of transcriptionally active vitellogenin II gene with the nuclear matrix of chicken liver.
1984,
Pubmed
Jost,
Estradiol-dependent trans-acting factor binds preferentially to a dyad-symmetry structure within the third intron of the avian vitellogenin gene.
1987,
Pubmed
Kadonaga,
Affinity purification of sequence-specific DNA binding proteins.
1986,
Pubmed
Kemler,
Octamer transcription factors bind to two different sequence motifs of the immunoglobulin heavy chain promoter.
1989,
Pubmed
Klein-Hitpass,
An estrogen-responsive element derived from the 5' flanking region of the Xenopus vitellogenin A2 gene functions in transfected human cells.
1986,
Pubmed
,
Xenbase
Saluz,
Genomic sequencing reveals a positive correlation between the kinetics of strand-specific DNA demethylation of the overlapping estradiol/glucocorticoid-receptor binding sites and the rate of avian vitellogenin mRNA synthesis.
1986,
Pubmed
Saluz,
Genomic sequencing and in vivo footprinting of an expression-specific DNase I-hypersensitive site of avian vitellogenin II promoter reveal a demethylation of a mCpG and a change in specific interactions of proteins with DNA.
1988,
Pubmed
Seiler-Tuyns,
Identification of estrogen-responsive DNA sequences by transient expression experiments in a human breast cancer cell line.
1986,
Pubmed
,
Xenbase
Spieth,
The C. elegans vitellogenin genes: short sequence repeats in the promoter regions and homology to the vertebrate genes.
1985,
Pubmed
,
Xenbase
Workman,
Transcriptional regulation by the immediate early protein of pseudorabies virus during in vitro nucleosome assembly.
1988,
Pubmed
,
Xenbase
