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Development 2004 Dec 01;13124:6107-17. doi: 10.1242/dev.01528.
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EDEN-BP-dependent post-transcriptional regulation of gene expression in Xenopus somitic segmentation.

Gautier-Courteille C , Le Clainche C , Barreau C , Audic Y , Graindorge A , Maniey D , Osborne HB , Paillard L .

EDEN-BP is a Xenopus RNA-binding protein that triggers deadenylation [poly(A) tail shortening], and thereby translational repression and degradation, of a subset of maternal mRNAs soon after fertilization. We show here that this factor is expressed in the presomitic mesoderm of older embryos, the site where somitic segmentation takes place. Inhibiting EDEN-BP function using either antisense morpholino oligonucleotides or neutralizing antibodies leads to severe defects in somitic segmentation, but not myotomal differentiation. This is associated with defects in the expression of segmentation markers belonging to the Notch signalling pathway in the presomitic mesoderm. We show by a combination of approaches that the mRNA encoding XSu(H), a protein that plays a central role in Notch signalling, is regulated by the EDEN-BP pathway. Accordingly, XSu(H) is overexpressed in EDEN-BP knock-down embryos, and overexpressing XSu(H) causes segmentation defects. We finally give data indicating that, in addition to XSu(H), other segmentation RNAs are a target for EDEN-BP. These results show that EDEN-BP-dependent post-transcriptional regulation of gene expression is required for the process of somitic segmentation.

PubMed ID: 15548579
Article link: Development

Species referenced: Xenopus laevis
Genes referenced: cdk1 celf1 dlc esr-5 hes5.6 hes5.7 myl4 notch1 rbpj
Morpholinos: celf1 MO1 celf1 MO2 rbpj MO1 rbpj MO5

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