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Methods Mol Biol
2019 Jan 01;1999:173-184. doi: 10.1007/978-1-4939-9500-4_10.
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Chromatin Immunoprecipitation (ChIP) of Plasmid-Bound Proteins in Xenopus Egg Extracts.
Wolfe KB
,
Long DT
.
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Xenopus egg extracts provide a cell-free system to analyze various aspects of chromatin biology. Here we describe a modified method of chromatin immunoprecipitation (ChIP) to detect the interaction of proteins with plasmid DNA incubated in extract. The combination of ChIP and Xenopus egg extracts provides a highly versatile and tractable approach to analyze dynamic protein-DNA interactions with great spatial and temporal detail.
Bernis,
Analysis of nuclear reconstitution, nuclear envelope assembly, and nuclear pore assembly using Xenopus in vitro assays.
2014, Pubmed,
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Bernis,
Analysis of nuclear reconstitution, nuclear envelope assembly, and nuclear pore assembly using Xenopus in vitro assays.
2014,
Pubmed
,
Xenbase
Collas,
The current state of chromatin immunoprecipitation.
2010,
Pubmed
Eisenhardt,
Xenopus in vitro assays to analyze the function of transmembrane nucleoporins and targeting of inner nuclear membrane proteins.
2014,
Pubmed
,
Xenbase
Fullbright,
p97 Promotes a Conserved Mechanism of Helicase Unloading during DNA Cross-Link Repair.
2016,
Pubmed
,
Xenbase
Gillespie,
Preparation and use of Xenopus egg extracts to study DNA replication and chromatin associated proteins.
2012,
Pubmed
,
Xenbase
Gilmour,
Detecting protein-DNA interactions in vivo: distribution of RNA polymerase on specific bacterial genes.
1984,
Pubmed
Gilmour,
In vivo interactions of RNA polymerase II with genes of Drosophila melanogaster.
1985,
Pubmed
Halpin,
Mitotic spindle assembly around RCC1-coated beads in Xenopus egg extracts.
2011,
Pubmed
,
Xenbase
Lebofsky,
DNA replication in nucleus-free Xenopus egg extracts.
2009,
Pubmed
,
Xenbase
Long,
Mechanism of RAD51-dependent DNA interstrand cross-link repair.
2011,
Pubmed
,
Xenbase
Long,
BRCA1 promotes unloading of the CMG helicase from a stalled DNA replication fork.
2014,
Pubmed
,
Xenbase
Park,
The MCM8-MCM9 complex promotes RAD51 recruitment at DNA damage sites to facilitate homologous recombination.
2013,
Pubmed
,
Xenbase
