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Mol Biol Rep
1987 Jan 01;124:265-71. doi: 10.1007/bf00444678.
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Biosynthesis of the neurofilament heavy subunit in Xenopus oocytes microinjected with rat brain poly(A)+ RNA.
Cross D
,
Allende ML
,
Krauss RY
,
Fuentes ME
,
Kaltwasser G
,
Alvarez J
,
Inestrosa NC
.
Abstract
In order to study the expression of the major subunit of neurofilaments (NFs), rat brain poly(A)+ RNA was purified by three different procedures and was injected in Xenopus laevis oocytes. This system was able to translate efficiently the 200 kDa NF subunit as shown by a dot-blot immunoassay and by immunoprecipitation of labeled NF polypeptides.
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972, Pubmed
Aviv,
Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
1972,
Pubmed
BARLOW,
A SIMPLE METHOD FOR THE QUANTITATIVE ISOLATION OF UNDEGRADED HIGH MOLECULAR WEIGHT RIBONUCLEIC ACID.
1963,
Pubmed
Berridge,
Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins.
1976,
Pubmed
,
Xenbase
Chirgwin,
Isolation of biologically active ribonucleic acid from sources enriched in ribonuclease.
1979,
Pubmed
Dahl,
Neurofilament phosphorylation in development. A sign of axonal maturation?
1986,
Pubmed
Delacourte,
Study of the 10-nm-filament fraction isolated during the standard microtubule preparation.
1980,
Pubmed
Dotto,
Signals for the initiation and termination of synthesis of the viral strand of bacteriophage f1.
1983,
Pubmed
Geisler,
Neurofilament architecture combines structural principles of intermediate filaments with carboxy-terminal extensions increasing in size between triplet proteins.
1983,
Pubmed
Gurdon,
Use of frog eggs and oocytes for the study of messenger RNA and its translation in living cells.
1971,
Pubmed
,
Xenbase
Hoffman,
Neurofilament gene expression: a major determinant of axonal caliber.
1987,
Pubmed
Jahn,
A quantitative dot-immunobinding assay for proteins using nitrocellulose membrane filters.
1984,
Pubmed
Laemmli,
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
1970,
Pubmed
Lasek,
Molecular biology of neuronal geometry: expression of neurofilament genes influences axonal diameter.
1983,
Pubmed
Lichy,
Isolation of proteins involved in the replication of adenoviral DNA in vitro.
1983,
Pubmed
Pachter,
The differential appearance of neurofilament triplet polypeptides in the developing rat optic nerve.
1984,
Pubmed
Palmiter,
Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.
1974,
Pubmed
Roberts,
Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.
1973,
Pubmed
Shaw,
Differential expression of neurofilament triplet proteins in brain development.
1982,
Pubmed
Shaw,
The immunological relatedness of neurofilament proteins of higher vertebrates.
1984,
Pubmed
Smart,
Synthesis of functional chick brain GABA-benzodiazepine-barbiturate/receptor complexes in mRNA-injected Xenopus oocytes.
1983,
Pubmed
,
Xenbase
Soreq,
The biosynthesis of biologically active proteins in mRNA-microinjected Xenopus oocytes.
1985,
Pubmed
,
Xenbase
Towbin,
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
1979,
Pubmed
Weber,
Neurofilaments, a subclass of intermediate filaments: structure and expression.
1983,
Pubmed
