Click here to close
Hello! We notice that you are using Internet Explorer, which is not supported by Xenbase and may cause the site to display incorrectly.
We suggest using a current version of Chrome,
FireFox, or Safari.
???displayArticle.abstract???
The vitellogenin gene is inactive in the liver of male Xenopus laevis, unless exogenous estrogen is administered. We have previously shown that conventional doses of estradiol-17beta result in the appearance of new hepatic messenger RNAs, some of which are encoded for vitellogenin. We now report that much higher doses of the hormone (2 mg/frog per day for 4 days) are required to elicit maximal responses. The relative levels of membrane-bound polysomes and vitellogenin mRNA were determined as a function of time and dose of hormone. Translation of total polysomal RNA in a cell-free system derived from wheat germ was used to estimate the relative levels of vitellogenin messenger RNA. Faithful translation of this messenger RNA was indicated by two lines of evidence: labeled cell-free products were immunoprecipitated with antivitellogenin antibody, and the migration of the major labeled product in sodium dodecyl sulfate/acrylamide gels was identical to that of native vitellogenin. Our results establish conditions for maximal estrogen-induced responses in this system, and are compatible with the hypothesis that a major regulatory mechanism of steroid hormones in the control of protein synthesis is that of gene activation and regulation of messenger RNA levels.
Ansari,
Chemical composition of an oestrogen-induced calcium-binding glycolipophosphoprotein in Xenopus laevis.
1971, Pubmed,
Xenbase
Ansari,
Chemical composition of an oestrogen-induced calcium-binding glycolipophosphoprotein in Xenopus laevis.
1971,
Pubmed
,
Xenbase
Bergink,
Precursor-product relationship between amphibian vitellogenin and the yolk proteins, lipovitellin and phosvitin.
1974,
Pubmed
,
Xenbase
Berridge,
Translation of Xenopus liver messenger RNA in Xenopus oocytes: vitellogenin synthesis and conversion to yolk platelet proteins.
1976,
Pubmed
,
Xenbase
Berridge,
Characterization of polysomes from Xenopus liver synthesizing vitellogenin and translation of vitellogenin and albumin messenger RNA's in vitro.
1976,
Pubmed
,
Xenbase
Blobel,
Ribosomes in rat liver: an estimate of the percentage of free and membrane-bound ribosomes interacting with messenger RNA in vivo.
1967,
Pubmed
FLECK,
The precision of ultraviolet absorption measurements in the Schmidt-Thannhauser procedure for nucleic acid estimation.
1962,
Pubmed
Follett,
The effects of oestrogen and gonadotrophins on lipid and protein metabolism in Xenopus laevis Daudin.
1968,
Pubmed
,
Xenbase
Ganoza,
In vitro synthesis of different categories of specific protein by membrane-bound and free ribosomes.
1969,
Pubmed
Heywood,
The identification of polyribosomes synthesizing myosin.
1967,
Pubmed
Lanclos,
Translation of hormone-induced messenger RNA in amphibian oocytes: I. Induction by estrogen of messenger RNA encoded for vitellogenic protein in the liver of the male African clawed toad (Xenopus laevis).
1975,
Pubmed
,
Xenbase
MOLLENHAUER,
PLASTIC EMBEDDING MIXTURES FOR USE IN ELECTRON MICROSCOPY.
1964,
Pubmed
MOORE,
ESTROGEN-INDUCED FORMATION OF UTERINE RIBOSOMES.
1964,
Pubmed
O'Malley,
Female steroid hormones and target cell nuclei.
1974,
Pubmed
Palmiter,
Magnesium precipitation of ribonucleoprotein complexes. Expedient techniques for the isolation of undergraded polysomes and messenger ribonucleic acid.
1974,
Pubmed
Palmiter,
Organization of polysomes from pre-existing ribosomes in chick oviduct by a secondary administration of either estradiol or progesterone.
1970,
Pubmed
Redshaw,
Comparative effects of the oestrogens and other steroid hormones on serum lipids and proteins in Xenopus laevis Daudin.
1969,
Pubmed
,
Xenbase
REYNOLDS,
The use of lead citrate at high pH as an electron-opaque stain in electron microscopy.
1963,
Pubmed
Roberts,
Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ.
1973,
Pubmed
Sarkar,
Isolation of messenger ribonucleic acid for myosin heavy chain.
1973,
Pubmed
Shapiro,
In vitro translation and estradiol-17beta induction of Xenopus laevis vitellogenin messenger RNA.
1976,
Pubmed
,
Xenbase
SIEKEVITZ,
A cytochemical study on the pancreas of the guinea pig. 5. In vivo incorporation of leucine-1-C14 into the chymotrypsinogen of various cell fractions.
1960,
Pubmed
Tata,
Co-ordination between membrane phospholipid synthesis and accelerated biosynthesis of cytoplasmic ribonucleic acid and protein.
1970,
Pubmed
Tata,
The expression of the vitellogenin gene.
1976,
Pubmed
,
Xenbase
Tata,
Ribonucleic acid synthesis during the early action of thyroid hormones.
1966,
Pubmed
Venkatesan,
Free and membrane-bound polysomes of rat liver: separation in nearly quantitative yield and analysis of structure and function.
1972,
Pubmed
Wahli,
Size, complexity and abundance of a specific poly(A)-containing RNA of liver from male Xenopus induced to vitellogenin synthesis by estrogen.
1976,
Pubmed
,
Xenbase
Wallace,
Resolution and isolation of avian and amphibian yolk-granule proteins using TEAE-cellulose.
1965,
Pubmed
Wallace,
Studies on amphibian yolk. VII. Serum phosphoprotein synthesis by vitellogenic females and estrogen-treated males of Xenopus laevis.
1968,
Pubmed
,
Xenbase
Weber,
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
1969,
Pubmed
Wittliff,
Regulation of yolk protein synthesis in amphibian liver. I. Induction of lipovitellin synthesis by estrogen.
1972,
Pubmed
,
Xenbase
