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XB-ART-31639
Nucleic Acids Res 1979 Aug 10;611:3685-701.
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Sequential Sepharose chromatographic isolation of polysomes and polysomal RNAs depleted in nuclear RNA from Xenopus.

Swiderski RE , Johnson SA , Larkins BA , Graham DE .


Abstract
Analyses of sequence complexities, stage specific gene expression, and mRNA sequence divergence require polysomal RNA preparations relatively free of nuclear RNA contamination. Conventional procedures for the isolation of uncontaminated polysomal RNAs which rely on sucrose density centrifugations are laborious and unsuitable for large scale isolations. We describe here a method using sequential Sepharose chromatography for isolating polysomes and polysomal RNAs depleted for nuclear RNA. Polysomes and polysomal RNAs isolated from livers of Xenopus stimulated to produce vitellogenin were capable of directing protein synthesis in vitro and showed little evidence of degradation. The polysomal RNAs contained less than 0.5% of nuclear RNA.

PubMed ID: 493118
PMC ID: PMC327966
Article link: Nucleic Acids Res



References [+] :
Angerer, Single copy DNA and structural gene sequence relationships among four sea urchin species. 1976, Pubmed